以RT-PCR为主题的“小综合”分子生物学实验课程设计与实践  

Design and Practice of"Small-scale Comprehensive"Molecular Biology Experiment Course with the Theme of RT-PCR

作　　者：迟彦[1] 王仁军[1] 王欢[1] 韩晓菲[1] 侯义龙[1] 曹阳[1] 付常振 CHI Yan;WANG Ren-jun;WANG Huan;HAN Xiao-fei;HOU Yi-long;CAO Yang;FU Chang-zhen(College of Life Science and Technology,Dalian University,Dalian,Liaoning 116622,China)

机构地区：[1]大连大学生命科学与技术学院,辽宁大连161622

出　　处：《教育教学论坛》2020年第23期200-201,共2页Education And Teaching Forum

基　　金：大连大学教改立项“虚拟仿真配套分子生物学实验教学体系的设计与实践”“‘互联网+’背景下学科平台课‘分子生物学’的混合式教学模式改革与实践”;2018年度辽宁省教育厅教改立项“基于双一流建设背景下生物技术专业课程体系的改革与探索”(辽教办〔2018〕165号)。

摘　　要：本着进一步完善和优化分子生物学实验课程的教学内容,加强本科生实验操作技能,提高本科生生物学综合创新思维能力,该文总结设计了以实时荧光定量PCR(Real-time PCR,RT-PCR)为主题的“小综合”实验,以期通过课程的实施,激发学生的学习兴趣,提高教学质量,取得良好的教学效果。In order to further improve and optimize the teaching content of Molecular Biology Experimental course,strengthen the experimental operation skills of undergraduates,and improve the comprehensive creative thinking ability of undergraduates,this article summarizes and designs a"small-scale comprehensive"experiment with the theme of RT-PCR.Through the implementation of the course,the students'learning interest is significantly stimulated,the teaching quality is improved,and a good teaching effect is achieved.

关 键 词：分子生物学实验 实时荧光定量PCR 综合实验 

分 类 号：G642[文化科学—高等教育学]