Fabrication of extracellular matrix-coated conductive polypyrrolepoly(L-lactide)fiber-films and their synergistic effect with(nerve growth factor)/(epidermal growth factor) on neurites growth  

作　　者：Ximing Pu Xingxing Zhou Zhongbing Huang Guangfu Yin Xianchun Chen 

机构地区：[1]College of Materials Science and Engineering,Sichuan University,Chengdu 610065,China

出　　处：《Chinese Chemical Letters》2020年第5期1141-1146,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.51273122);Sichuan Science and Technology Project(No.2018JY0535)。

摘　　要：Non-nerve cell-derived extracellular matrix(ECM)wa s coated on the aligned porous polypyrrole-poly(Llactide)(PPy-PLLA)fiber-films with the conductivity of^12 mS/m via L929 cells culture and lysing,resulting in^10%increase of PC12 cells attachment and^26μm increase of neurites length.The neurite length of^149μm in EGF/NGF group(optimal concentration radio of 12.5/50(ng/mL))on aligned and ECM-conjugated fiber-films was significantly larger than^94μm in only NGF group(50 ng/mL),confirming the synergy of EGF,NGF and aligned ECM-conjuaged PPy-PLLA fibers.When differentiated PC12 cells were exerted electrical stimulation(ES)of 100 mV/cm for 4 h/day in 2 day through ECM-PPyPLLA fiber-films,their neurite length reached to^251μm,significantly larger than^149μm of group without ES,due to the higer expression of related neural proteins in ES group.A simple mechanism was proposed to analyze synergistical effect of ECM,EGF,NGF on axons adhesion and elongation along the aligned ECM-coated fibers under ES condition.

关 键 词：Extracellular matrix Axonal growth Conducting PPy-PLLA fiber-film Nerve growth factor Epidermal growth factor Electrical stimulation 

分 类 号：R318.08[医药卫生—生物医学工程]