基于丝网印刷碳纳米管电极的电化学传感检测有机磷农药  

Commercial screen-printed carbon-nanotube electrode-based electrochemical biosensor for the detection of organophosphorus pesticide

作　　者：梁刚[1,2,3] 张全刚 赵杰 靳欣欣 潘立刚[1,2,3] LIANG Gang;ZHANG Quangang;ZHAO Jie;JIN Xinxin;PAN Ligang(Beijing Academy of Agriculture and Forestry Science,Beijing Research Center for Agricultural Standards and Testing,Beijing100097;Risk Assessment Lab for Agro-products(Beijing),Beijing100097;Beijing Municipal Key Laboratory of Agriculture Environment Monitoring,Beijing100097;Beijing Shoufa Tianren Ecological Landscape Co.,Ltd.,Beijing 105600)

机构地区：[1]北京市农林科学院,北京农业质量标准与检测技术研究中心,北京100097 [2]农业部农产品质量安全风险评估实验室(北京),北京100097 [3]农产品产地环境监测北京市重点实验室,北京100097 [4]北京市首发天人生态景观有限公司,Beijing 105600

出　　处：《分析试验室》2020年第6期636-639,共4页Chinese Journal of Analysis Laboratory

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20170420);国家自然科学基金青年基金(21806013);北京市自然科学基金(L182031);北京市优秀人才青年骨干个人项目(2017000020060G127)资助。

摘　　要：采用商品化丝网印刷碳纳米管电极(CNTSPE),并利用层层组装技术将乙酰胆碱酯酶(AChE)、聚二烯丙基二甲基氯化铵(PDDA)逐层依次修饰于CNTSPE表面,制备了PDDA/AChE/PDDA/AChE/PDDA修饰的CNTSPE传感器(PDDA/(AChE/PDDA)2/CNTSPE),并以对氧磷、毒死蜱为研究模型,考察了该电化学传感器的性能并建立了对氧磷、毒死蜱的电化学传感检测方法。结果表明,电极对氧磷、毒死蜱两种农药的线性响应范围分别为50~150 ng/mL,20~150 ng/mL,检测限分别为20 ng/mL,10 ng/mL。该传感器应用于实际样品中目标农药的检测,回收率在87. 5%~90. 6%之间。By using the commercial screen-printed carbon-nanotube electrode(CNTSPE)as the solid electrode,acetylcholinesterase(AChE)and poly dimethyl diallyl ammonium chloride(PDDA)were sequentially self-assembled onto the CNTSPE film,obtaining the electrochemical PDDA/(AChE/PDDA)2/CNTSPE biosensor.Taking paraoxon and chlorpyrifos as the model analytes,the detection performance of the biosensor was investigated,and the detectionmethods were established.Results showed that the linear response ranges for the determination of paraoxon and chlorpyrifos were 40 to 200 ng/mL and 10 to 150 ng/mL,respectively.The corresponding detection limits were 20 ng/mL and 10 ng/mL,respectively.The sensor was applied to analysis of paraoxon and chlorpyrifos spiked into tap water with recoveries of 87.5%-90.6%,indicating that the assay could be applied to real sample analysis.

关 键 词：农药 丝网印刷电极 电化学 传感器 

分 类 号：O657.1[理学—分析化学]