刺五加总苷及刺五加苷B对持续黑暗条件下果蝇睡眠-觉醒节律的量效关系研究  

Study on the dose-effect relationship of acanthopanax total glycoside and eleutheroside B on sleep-wake rhythm in drosophila melanogaster under continuous darkness

作　　者：卞宏生[1] 蔡江莹 杨凡静暄 BIAN Hongsheng;CAI Jiangying;YANG Fanjingxuan(Heilongjiang University of Chinese Medicine,Harbin,150040,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国药物依赖性杂志》2020年第3期231-236,共6页Chinese Journal of Drug Dependence

基　　金：国家自然科学基金面上项目(87214114);教育部重点实验室---北药基础与应用研究开放课题基金资助;黑龙江中医药大学校科研基金资助;黑龙江中医药大学博士创新基金(2015bs08);黑龙江中医药大学科研基金(201101)。

摘　　要：目的:探讨刺五加总苷及苷B改善睡眠-觉醒节律的量效关系。方法:以果蝇为实验对象,在持续黑暗条件下,总苷固定给药7 d,苷B给药3 d,采用DAMS监测果蝇活动及睡眠状态,分析比较其平均睡眠时间、睡眠-觉醒节律和睡眠时间百分比。结果:刺五加总苷对于果蝇睡眠时间表现低剂量增加(P<0.05)高剂量减少(P<0.05),增加片段化睡眠,略微增多白天睡眠时间百分比。刺五加苷B可通过增加果蝇夜晚睡眠时间(P<0.001)进而延长全天睡眠时间(P<0.01),增加片段化睡眠,增多白天及夜晚睡眠时间百分比。结论:刺五加总苷、苷B均能改善果蝇睡眠,其最佳剂量为0.125%(即生药量7.125 mg·kg^-1)总苷、1%苷B。Objective:This study aims to investigate the dose-effect relationship of acanthopanax senticosus and eleutheroside B in improving sleep-wake rhythm.Methods:Drosophila melanogaster was administered with acanthopanax senticosus for 7 days and eleutheroside B for 3 days under the condition of darkness,and the activity and sleep state were monitored by DAMS.After that,the sleep time,sleep-wake rhythm and percentage of sleep time were analyzed and compared.Results:Decreased acanthopanax senticosus led to more sleep time of drosophila melanogaster(P<0.05),and increased dose resulted in less sleep time(P<0.05).This also increased the fragmented sleep and slightly increased the percentage of daytime sleep time.Eleutheroside B could prolong the entire sleep time(P<0.001)by increasing the night sleep time(P<0.001),so as to increase the fragmented sleep time and the percentage of the day and night sleep time.Conclusion:Acanthopanax senticosus and eleutheroside B can improve the sleep of drosophila melanogaster.The optimum dosage is 0.125%(dried medicinal herbs is 7.125 mg·kg^-1)of acanthopanax senticosus and 1%of eleutheroside B.

关 键 词：刺五加总苷 刺五加苷B 黑腹果蝇 睡眠-觉醒节律 量效关系 

分 类 号：Q428[生物学—神经生物学]