柱前衍生高效液相色谱法测定山黧豆中β-二氨基丙酸的方法研究  

Determination of Toxin β-Diaminopropionic Acid in Lathyrussativuswith Pre-Column Derivatization High Performance Liquid Cromalography

作　　者：张小寒 董晓宁 杨玲娟 Zhang Xiaohan;Dong Xiaoning;Yang Lingjuan(School of Chemical Engineering&Technology,Tianshui Normal Universily,Tianshui,Gansu 741001,China;College of Food Science&Nutritional Engineering,China Agricultural University,Bejing 100083,China)

机构地区：[1]天水师范学院化学工程与技术学院,甘肃天水741001 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《化学世界》2020年第6期416-420,共5页Chemical World

基　　金：国家自然科学基金(Nos.31160060,31260568);天水师范学院中青年科学基金(No.TSA1301)资助项目。

摘　　要：建立了柱前衍生高效液相色谱法测定山黧豆中β-二氨基丙酸(β-ODAP)含量的方法。采用Luna-C18色谱柱(4.6mm×250mm,5μm),以乙腈和HAc-NaAc为流动相(17∶83,V∶V),λ=360nm,柱温40℃,取20μL进样分析。β-ODAP在0.39~25.27μg/mL范围,其吸收峰峰面积与其质量浓度线性关系良好,A=140.50c+72.30,R=0.999 5;山黧豆种子中β-ODAP的含量最高。方法操作简便、快速、准确,选择性好,可用于植物样中β-ODAP含量的测定。A pre-column derivatization high performance liquid cromalography(HPLC) method was established for the determination ofβ-diaminoprionic acid(β-ODAP)in Lathyrus sativus.Luna-C18 column(4.6 mm×250 mm,5μm)was used with acetonitrile and HAc-NaAc as mobile phase(17:83,λ=360 nm,t=40℃,20μL sample).In the range of 0.39~25.27μg/mL,this method has a good linear relationship(A=140.50 c+72.30,R=0.999 5).The contents ofβ-ODAP in different parts(seeds,roots,stems,leaves)of Lathyrus sativus were different and the highest content is in seeds.The method is simple,rapid,accurate and selective so that it can be used for the determination ofβ-ODAP in plant samples.

关 键 词：β-ODAP 高效液相色谱 2 4-二硝基氟苯 山黧豆 

分 类 号：O641.4[理学—物理化学]