五味子乙素对阿帕替尼抗肝癌细胞的增强作用  

Enhancement of schisandrin B on apatinib against hepatoma

作　　者：朱彤[1] 饶井芬 刘承一[1] 肇爽[1] 张力[1] ZHU Tong;RAO Jing-fen;LIU Cheng-yi;ZHAO Shuang;ZHANG Li(Department of Oncology,Affiliated Hospital of Chengde Medical College,Chengde 067000,Hebei Province,China)

机构地区：[1]承德医学院附属医院肿瘤科,河北承德067000

出　　处：《中国临床药理学杂志》2020年第11期1503-1506,共4页The Chinese Journal of Clinical Pharmacology

基　　金：承德市科学技术研究与发展计划基金资助项目(201706A039)。

摘　　要：目的探索五味子乙素对阿帕替尼抗肝癌细胞活性的影响及其分子机制。方法将体外培养的肝癌细胞分为8组:空白组(不做任何处理)、五味子乙素组(10μmol·L^-1五味子乙素)、阿帕替尼组(30μmol·L^-1阿帕替尼)和第一联合组至第五联合组[5个浓度(5,8,10,15,20μmol·L^-1)五味子乙素组+30μmol·L^-1阿帕替尼]。用CCK-8实验检测细胞存活率,根据细胞存活率选用第三联合组进行后续实验。用流式细胞术实验检测细胞凋亡情况;用细胞划痕实验检测细胞迁移情况;用蛋白质印迹法检测相关蛋白的表达。结果空白组、五味子乙素组、阿帕替尼组和第三联合组的Hep3B细胞存活率分别为(100.00±1.36)%,(96.82±1.94)%,(88.43±0.82)%和(57.54±0.91)%;这4组的凋亡细胞率分别为(4.61±0.28)%,(12.62±1.94)%,(16.13±1.37)%和(48.84±2.18)%;这4组的细胞移行愈合率分别为(90.91±2.41)%,(59.42±2.66)%,(45.43±2.58)%和(25.24±1.93)%;这4组磷酸化丝裂原活化蛋白激酶P38(p-P38AMPK)的相对表达量为0.11±0.02,0.22±0.04,0.33±0.07和1.14±0.11;这4组的磷酸化丝裂原活化蛋白激酶(p-JNK)蛋白的相对表达量为0.21±0.05,0.22±0.08,0.73±0.08和1.14±0.14。上述指标:第三联合组与阿帕替尼组比较,差异均有统计学意义(均P<0.001)。结论五味子乙素可能通过P38/JNK信号通路增强阿帕替尼对人肝癌细胞的抗肿瘤活性。Objective To investigate the effect of schisandrin B on the activity of apatinib against liver cancer cells and its molecular mechanism.Methods Hepatocellular carcinoma cells were divided into eight groups:blank group(did not do any treatment),schisandrin B group(10μmol·L^-1 schisandrin B),apatinib group(30μmol·L^-1 apatinib)and combination-1,-2,-3,-4,-5 groups[five concentrations(5,8,10,15,20μmol·L^-1)schisandrin B+30μmol·L^-1 apatinib].CCK-8 assay was used to detect cell survival rate.According to the cell survival rate,the combination-3 group was selected for the follow-up experiment.Flow cytometry was used to detect apoptosis.Cell scratch was used to detect cell migration.Western blotting was used to detect the expression of related proteins.Results The cell survival rates in blank group,schisandrin B group,apatinib group and combination-3 group were(100.00±1.36)%,(96.82±1.94)%,(88.43±0.82)%and(57.54±0.91)%;the apoptotic rates in the four groups were(4.61±0.28)%,(12.62±1.94)%,(16.13±1.37)%and(48.84±2.18)%;the transition healing rates in the four groups were(90.91±2.41)%,(59.42±2.66)%,(45.43±2.58)%and(25.24±1.93)%;the relative expression levels of phosphorylated P38 mitogen-activated protein kinase(p-P38 AMPK)in the four groups were 0.11±0.02,0.22±0.04,0.33±0.07 and 1.14±0.11;the relative expression levels of phosphorylated mitogen-activated protein kinase(p-JNK)protein in the four groups were 0.21±0.05,0.22±0.08,0.73±0.08 and 1.14±0.14.Comparison between combination-3 group and apatinib group,the difference of the factors were significant(all P<0.01).Couclosion Schisandrin B may enhance the antitumor activity of apatinib against human liver cancer cells through the P38/JNK signaling pathway.

关 键 词：五味子乙素 人肝癌细胞 阿帕替尼 细胞迁移 细胞凋亡 

分 类 号：R28[医药卫生—中药学]