他莫昔芬对不同乳腺癌细胞株的影响  

Effect of tamoxifen on different breast cancer cell lines

作　　者：李啸天 陈欢 杨光伦[1] LI Xiao-tian;CHEN Huan;YANG Guang-lun(Department of Endocrinology and Breast Surgery,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学附属第一医院内分泌乳腺外科,重庆400016

出　　处：《中国临床药理学杂志》2020年第11期1518-1520,1539,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81072149)。

摘　　要：目的探讨他莫昔芬对人乳腺癌细胞株MCF-7、SKBR3和MDA-MB-231细胞增殖的影响。方法取人乳腺癌细胞株MCF-7、SKBR3和MDA-MB-231细胞进行实验,其中增殖抑制实验给予0.10,0.20,0.50和1.00μg·mL^-1他莫昔芬分别干预24,48和72 h;凋亡实验和蛋白检测均给予1.00μg·mL^-1他莫昔芬分别干预72 h和24 h。用噻唑蓝法检测各组细胞的抑制情况,用流式细胞仪检测各细胞的凋亡率,用Western Blot检测各细胞Bcl-2和Fas蛋白的表达水平。结果随着他莫昔芬浓度升高和干预时间延长,他莫昔芬对MCF-7、SKBR3和MDA-MB-231细胞抑制作用逐渐增强,其中对MCF-7和SKBR3细胞的抑制作用均显著强于MDA-MB-231细胞。MCF-7和SKBR3细胞的凋亡率分别为(41.15±5.01)%和(42.28±8.03)%,明显高于MDA-MB-231细胞的(9.24±3.31)%,差异均有统计学意义(均P<0.05)。干预24 h后,MCF-7和SKBR3细胞的Fas蛋白相对表达量分别为(0.978±0.106)和(0.980±0.110)均明显高于MDA-MB-231细胞的(0.654±0.108),Bcl-2蛋白相对表达量分别为(0.351±0.106)和(0.349±0.112)均明显低于MDA-MB-231细胞的(0.641±0.109),差异均有统计学意义(均P<0.05)。结论他莫昔芬能有效抑制人乳腺癌细胞株MCF-7、SKBR3和MDA-MB-231的增殖,促进细胞凋亡,存在时间-剂量效应,且对MCF-7和SKBR3细胞的影响强于MDA-MB-231细胞。Objective To investigate the effect of tamoxifen on human breast cancer cell lines MCF-7,SKBR3 and MDA-MB-231.Methods Human breast cancer cell lines MCF-7,SKBR3 and MDA-MB-231 were taken for experiment.Proliferation inhibition experiment was given 0.10,0.20,0.50 and 1.00μg·mL^-1 tamoxifen for 24,48 and 72 hours,respectively.Apoptosis test and protein detection were treated with 1.00μg·mL^-1 tamoxifen for 72 h and 24 h,respectively.The inhibition of cells was detected by thiazolyl blue method,the apoptosis rate was detected by flow cytometry,and the expression levels of Bcl-2 and Fas protein were detected by Western Blot.Results With the increase of concentration and time of tamoxifen,the inhibitory effects of tamoxifen on MCF-7,SKBR3 and MDA-MB-231 cells were enhanced gradually,and the inhibitory effects of MCF-7 and SKBR3 cells were significantly stronger than that of MDA-MB-231 cells.The apoptosis rates of MCF-7 and SKBR3 cells were(41.15±5.01)%and(42.28±8.03)%,respectively,which were significantly higher than those of MDA-MB-231 cells(9.24±3.31)%,the differences were statistically significant(all P<0.05).After intervention for 24 hours,the relative expressions of Fas protein in MCF-7 and SKBR3 cells(0.978±0.106)and(0.980±0.110)were significantly higher than that in MDA-MB-231 cells(0.654±0.108),while the relative expressions of Bcl-2 protein in MDA-MB-231 cells(0.351±0.106)and(0.349±0.112)was significantly lower than that in MDA-MB-231 cells(0.641±0.109),the differences were statistically significant(all P<0.05).Conclusion Tamoxifen can effectively inhibit the proliferation of human breast cancer cell lines MCF-7,SKBR3 and MDA-MB-231,promote apoptosis in a time-dose effect,and the effect on MCF-7 and SKBR3 cells is stronger than that on MDA-MB-231 cells.

关 键 词：他莫昔芬 人乳腺癌细胞株 MCF-7细胞 SKBR3细胞 MDA-MB-231细胞 增殖 凋亡 

分 类 号：R979.1[医药卫生—药品]