丙泊酚镇静对大鼠认知功能的影响  

Effects of propofol sedation on cognitive function in rats

作　　者：黄海娟[1] 陆辉 HUANG Hai-juan;LU Hui(Department of Anesthesiology,Xiamen Traditional Chinese Medicine Hospital,Xiamen 361000,Fujian Province,China;Department of Anesthesiology,Xiamen Haicang Hospital,Xiamen 361026,Fujian Province,China)

机构地区：[1]厦门市中医院麻醉科,福建厦门361000 [2]厦门市海沧医院麻醉科,福建厦门361026

出　　处：《中国临床药理学杂志》2020年第11期1552-1554,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究丙泊酚镇静对大鼠认知功能及海马组织脑源性神经生长因子-酪氨酸激酶-B(BDNF-Trk-B)信号通路的影响。方法将SPF级、雄性Wistar大鼠60只,随机分为对照组(n=20)、脂肪乳组(n=20)及实验组(n=20)。对照组腹腔注射等量生理盐水;脂肪乳组腹腔注射5%脂肪乳剂(100 mg·kg^-1),每20 min给予首次剂量的1/2;实验组腹腔注射100 mg·kg^-1丙泊酚注射液(10 mg·mL^-1),每20 min给予首次剂量的1/2,麻醉维持2 h。干预4 h后,用Morris水迷宫测试仪检测大鼠空间学习记忆能力;以苏木精-伊红(HE)染色观察大鼠海马组织病理学变化;以蛋白质印迹(Wb)法检测大鼠海马组织中脑源性神经生长因子(BDNF)和酪氨酸激酶-B(Trk-B)蛋白表达。结果对照组、脂肪乳组及实验组大鼠第4天逃逸潜伏期分别为(18.28±16.32),(18.96±16.51),(72.78±35.42)s;各组大鼠的空间探索时间分别为(35.24±12.49),(35.19±12.43),(23.27±4.82)s;穿越平台次数分别为(4.91±2.14),(4.83±2.12),(0.67±0.74)次;海马组织BDNF蛋白相对表达量分别为0.83±0.12,0.79±0.11,0.17±0.03;Trk-B蛋白相对表达量分别为0.89±0.13,0.87±0.11,0.26±0.04。与对照组和脂肪乳组相比,实验组大鼠第1~4天逃逸潜伏期均明显延长,空间探索时间显著缩短,穿越平台次数减少;且大鼠海马组织中BDNF和Trk-B蛋白表达显著降低(均P<0.05)。结论丙泊酚可导致大鼠海马组织发生病理学变化,其可抑制海马组织BDNF-Trk-B信号通路活性,导致海马结构和功能受损,最终影响认知功能。Objective To investigate the effects of propofol sedation on cognitive function and brain-derived nerve growth factor-tyrosine kinase-B(BDNF-Trk-B)signaling pathway in hippocampus of rats.Methods Sixty SPF and male Wistar rats were randomly divided into control group(n=20),fat emulsion group(n=20)and test group(n=20).Control group was intraperitoneally injected with the same amount of normal saline;fat emulsion group was intraperitoneally injected with 5%fat emulsion 100 mg·kg-,and 1/2 of the first dose was administered every 20 min;test group was intraperitoneally injected with 100 mg·kg^-1 propofol injection 10 mg·mL^-1,and 1/2 of the first dose was administered every 20 min,and the anesthesia was maintained for 2 h.After 4 h of intervention,the spatial learning and memory ability of rats was detected by Morris water maze test;hematoxylin-eosin(HE)staining was used to observe the pathological changes of hippocampus in rats;Western blot(Wb)assay was used to detect brain-derived nerve growth factor(BDNF)and tyrosine kinase-B(Trk-B)protein expression in rat hippocampus.Results The escape latency of control group,fat emulsion group and test group on the fourth day were(18.28±16.32),(18.96±16.51),(72.78±35.42)s;the spatial exploration time of each group of rats were(35.24±12.49),(35.19±12.43),(23.27±4.82)s;the number of crossing platforms were(4.91±2.14),(4.83±2.12)and(0.67±0.74)times;the relative expression levels of BDNF protein in hippocampus were 0.83±0.12,0.79±0.11 and 0.17±0.03;the relative expression levels of Trk-B protein were 0.89±0.13,0.87±0.11 and 0.26±0.04.Compared with control group and fat emulsion group,the escape latency of test group were significantly prolonged from day 1 to day 4,the space exploration time was significantly shortened,and the number of crossing platforms was significantly reduced;and the expression levels of BDNF and Trk-B protein in rat hippocampus were significantly decreased(all P<0.05).Conclusion Propofol can cause pathological changes in hippocampus of rats,

关 键 词：丙泊酚 认知功能 海马组织 脑源性神经生长因子-酪氨酸激酶-B 信号通路 

分 类 号：R971.2[医药卫生—药品]