大鼠成纤维细胞同步处理的流式细胞仪分析  

Flow Cytometric Cell Cycle Analysis of Synchronization treated Rat Fibroblast Cells

作　　者：刘志春[1] 李六金[1] 杨贵忠[1] 冯秀亮[2] 谢松涛[1] 朱华萍[1] 张生勇[1] 

机构地区：[1]第四军医大学实验动物中心陕西西安710032 [2]西北农林科技大学陕西杨陵712100

出　　处：《黄牛杂志》2002年第6期16-18,共3页Journal of Yellow Cattle Science

摘　　要：目的 :研究 SD大鼠成纤维细胞同步处理后的各细胞周期的变化 ,探讨供体细胞的细胞周期对核移植胚胎发育的影响。方法 :分离培养 SD大鼠皮肤成纤维细胞 ,传 3～ 5代 ,分别经血清饥饿 2～ 6d和 1 0 mg/ ml nocodazole处理 2 4 h,流式细胞仪分析处理组和对照组的细胞周期百分比。结果 :血清饥饿、nocodazole处理及对照组的 G0 + G1期成纤维细胞的百分比 ( % )分别为 74.5± 4.4、49.5± 4.65和 5 0 .1 2± 3.9;G2 / M期细胞的百分比 ( % )分别为 :1 9.2 5± 4.2、2 5 .98± 2 .7及 45 .9± 5 .2 1。结论 :血清饥饿能较好地使大鼠成纤维细胞同步于 G0 和 G1期 ;nocodazole处理则使细胞较大程度同步于 G2 和 M期。To investigate the cell cycle characteristics of SD rat fibroblast cells cultured under starvation of nocodazole synchronization treatment. After 3~5 passages of the primary fibroblast cells, the cells were starvated with serum for 2~5 days or treated with 10 mg/ml nocodazole for 24 hours, then calculate the percentage of the treated cells and the control cells in G 0, G 1 and G 2+M phases of the cell cycle by using flow cytometry respectively. Results showed that G 0, +G 1 cell percentage of the serum starvation, nocodazole treatment, and control were 74.5±4.4,49.5±4.65 and 50.12±3.9, respectively, G 2+M cells were 19.25±4.2,25.98±2.7 and 45.9±5.21, which showed rat fibroblast cells treated with nocodazole for 5 days contained higher (P<0.05) percentage of G 0, +G 1 cell and cells treated with nocodazole contained higher percentage of G 2+M cells.

关 键 词：大鼠 成纤维细胞 同步处理 核移植 流式细胞仪分析 

分 类 号：Q813[生物学—生物工程] Q954.4