产气荚膜梭菌ε毒素导致多器官组织损伤  

Multiple organ damage induced by Clostridium perfringens ε toxin

作　　者：耿志军 辛文文 黄静 康琳 袁媛 王景林[1,2] GENG Zhi-Jun;XIN Wen-Wen;HUANG Jing;KANG Lin;YUAN Yuan;WANG Jing-Lin(Graduate School of Anhui Medical University,Hefei,Anhui 230032,China;State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,china)

机构地区：[1]安徽医科大学研究生院,安徽合肥230032 [2]军事医学研究院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071

出　　处：《微生物学通报》2020年第6期1920-1926,共7页Microbiology China

基　　金：国家重点研发计划(2017YFC1200900)。

摘　　要：【背景】产气荚膜梭菌ε毒素(εtoxin,ETX)是由B型和D型产气荚膜梭菌产生的一种肠毒素,具有迅速的致命性和经济上的破坏性。【目的】研究ETX对小鼠多个脏器的损伤情况及其在不同小肠肠段的结合情况。【方法】构建纯化偶联红色荧光蛋白的mScarlet-ETX,使用小动物活体成像观察其在小鼠体内的累积情况;使用病理学技术观察ε毒素对小鼠各个脏器和不同肠段的结合情况,以及对脏器的损伤情况。【结果】产气荚膜梭菌感染后,产生的ETX能累积于脑、肾、肺、肝、脾和心脏组织中,并能导致这些脏器的损伤。ETX在小鼠的小肠中主要累积于结肠中。【结论】产气荚膜梭菌ε毒素导致多器官组织损伤,并主要在结肠上被吸收,为治疗和预防携带ε毒素的产气荚膜梭菌感染提供了理论依据。[Background]Clostridium perfringensεtoxin(ETX)is an enterotoxin produced by C.perfringens type B and D,which is rapidly fatal and economically destructive.[Objective]Our study aimed to investigate the damage of ETX on multiple organs in mice and the difference of binding capability in different intestinal segments.[Methods]The m Scarlet-ETX coupled with red fluorescence was constructed and visualized with in vivo imaging system to observe its accumulation in vivo.The combination ofεtoxin on various organs and different intestinal segments of mice and the damage to organs were analyzed by pathological sectioning.[Results]It was found that after infection with C.perfringens,the produced ETX could accumulate in the brain,kidney,lung,liver,spleen,heart tissues and caused damage to these organs.ETX in the small intestine of mice was mainly accumulated in the colon.[Conclusion]These results indicated that C.perfringensεtoxin could cause damage to multiple organs and mainly absorbed in the colon,which provided a theoretical basis for the treatment and prevention of C.perfringens containing the epsilon toxin gene infection.

关 键 词：产气荚膜梭菌 ε毒素 器官损伤 出血 水肿 

分 类 号：S852.61[农业科学—基础兽医学]