Dual roles of juvenile hormone signaling during early oogenesis in Drosophila  

作　　者：Wei Luo Sethuraman Veeran Jian Wang Sheng Li Kang Li Su-Ning Liu 

机构地区：[1]Guangzhou Key Laboratory of Insect Development Regulation and Application Research,Institute of Insect Science and Technology&School of Life Sciences,South China Normal University,Guangzhou,China and 2 Department of Entomology,University of Maryland,College Park,MD,USA

出　　处：《Insect Science》2020年第4期665-674,共10页昆虫科学（英文版）

基　　金：We thank Dr.Jian-Hua Huang for flies and helpful comments on this manuscript.This work was supported by the National Natural Science Foundation of China(Grant No.31702054).

摘　　要：Juvenile hormone(JH)signaling plays crucial roles in insect metamorphosis and reproduction.Function of JH signaling in germline stem cells(GSCs)remains largely unknown.Here,we found that the number of GSCs significantly declined in the ovaries of Met,Gee and JHAMT mutants.Then we inhibited JH signaling in selected cell types of ovaries by expressing Met and Gee or Kr-hl double-stranded RNAs(dsRNAs)using different Gal4 drivers.Blocking of JH signaling in muscle cells has no effect on GSC numbers.Blocking of JH signaling in cap cells reduced GSCs cells.Inductive expression of Met and Gee dsRNA but not Kr-hl by Nos-Gal4 increased GSC cells.These results indicate that JH signaling plays an important role in GSC maintenance.

关 键 词：cap cells germline stem cells juvenile hormone OOGENESIS OVARY 

分 类 号：Q96[生物学—昆虫学]