Hedgehog promotes cell proliferation in the midgut of silkworm,Bombyx mori  

作　　者：Shun-Qin Zhu Ya-Jun Zhang Muhammad Nadeem Abbas Xiang-Wei Hao Yu-Zu Zhao Hang-Hua Liang Hong-Juan Cui Li-Qun Yang 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing,China [2]School of Life Sciences,Southwest University,Chongqing,China [3]Chongqing Engineering Laboratory of Targeted and Innovative Therapeutics,Chongqing Key Laboratory of Kinase Modulators as Innovative Medicine,IATTI,Chongqing University of Arts and Sciences,Chongqing,China

出　　处：《Insect Science》2020年第4期697-707,共11页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Sci-ence Foundation of China(No.31672496);the Natural Science Foundation of Chongqing(cstc2016jcyjA0425);the Chongqing University Innovation Team Building Program funded projects(CXTDX201601010);the Fundamental Research Funds for the Central Universi-ties(No.XDJK2015C129,No.2362015XK09,No.XDJK2013B020 and No.20120524);and the Scientific Research Foundation of the Chongqing University of Arts and Sciences(2017ZBX10).

摘　　要：The Hedgehog(Hh)signaling pathway is one o f the major regulators of embryonic development and tissue homeostasis in multicellular organisms.However,the role of this pathway in the silkworm,especially in the silkworm midgut,remains poorly understood.Here,we report that Bombyx mori Hedgehog(BmHh)is expressed in most tissues of silkworm larvae and that its functions are well-conserved throughout evolution.We further demonstrate that the messenger RNA o f four Hh signaling components,BmHh ligand,BmPtch receptor,signal transducer BmSmo and transcription factor BmCi,are all upregulated following Escherichia coli or Bacillus thuringiensis infection,indicating the activation o f the Hh pathway.Simultaneously,midgut cell proliferation is strongly promoted.Conversely,the repression of Hh signal transduction with double-stranded RNA or cyclopamine inhibits the expression o f BmHh and BmCi and reduces cell proliferation.Overall,these findings provide new insights into the Hh signaling pathway in the silkworm,B.mori.

关 键 词：cell proliferation CYCLOPAMINE Hedgehog midgut SILKWORM 

分 类 号：Q96[生物学—昆虫学]