Identification of the ryanodine receptor mutation I4743M and its contribution to diamide insecticide resistance in Spodoptera exigua (Lepidoptera: Noctuidae)  

作　　者：Ya-Yun Zuo Huan-Huan Ma Wen-Jie Lu Xing-Liang Wang Shu-Wen Wu Ralf Nauen Yi-Dong Wu Yi-Hua Yang 

机构地区：[1]College of Plant Protection,Nanjing Agricultural University,Nanjing,China [2]Bayer CropScience,R&D Pest Control,Monheim,Germany

出　　处：《Insect Science》2020年第4期791-800,共10页昆虫科学（英文版）

基　　金：This work was funded by a grant from the Ministry of Agriculture and Rural Affairs(MARA)of China(No.2016YFD0200500).

摘　　要：Insect ryanodine receptors(RyRs)are the targets of diamide insecticides.Two point mutations G4946E and I4790M(numbering according to Plutella xylostella,PxRyR)in the transmembrane domain of the insect RyRs associated with diamide resistance have so far been identified in three lepidopteran pests,P.xylostella,Tuta absoluta and Chilo suppressalis.In this study,we identified one of the known RyR target site resistance mutations(I4790M)in a field-collected population of Spodoptera exigua.The field-collected WF population of S.exigua exhibited 154 fold resistance to chlorantraniliprole when compared with the susceptible WH-S strain.Sequencing the transmembrane domains of S.exigua RyR(SeRyR)revealed that the resistant WF strain was homozygous for the 14743M mutation(corresponding to I4790M in PxRyR),whereas the G4900E allele(corresponding to G4946E of PxRyR)was not detected.The 4743M allele was introgressed into the susceptible WH-S strain by crossing WF with WH-S,followed by three rounds of backcrossing with WH-S.The introgressed strain 4743M was homozygous for the mutant 4743M allele and shared about 94%of its genetic background with that of the recipient WH-S strain.Compared with WH-S,the near-isogenic 4743M strain showed moderate levels of resistance to chlorantraniliprole(21 fold),cyantraniliprole(25 fold)and flubendiamide(22 fold),suggesting that the I4743M mutation confers medium levels of resistance to all three diamides.Genetic analysis showed diamide resistance in the 4743M strain was inherited as an autosomal and recessive trait.Results from this study have direct implications for the design of appropriate resistance monitoring and management practices to sustainably control S.exigua.

关 键 词：beet armyworm diamide insecticides point mutation resistance ryanodine receptor 

分 类 号：Q96[生物学—昆虫学]