UPLC同时测定铁棒锤中6种酯型生物碱类成分的含量  

UPLC Siultaneous Determination of Six Esteric Alkaloids Components in Aconitum Flaram Hand.Mazz

作　　者：张妍[1,2] 付雪艳[1] Zhang Yan;Fu Xueyan(Ning xia Medical University,Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine,Ningxia,750001,China;Ning Xia Medical University,Key Laboratory of Hui Ethnic Medicine Modernization,Ministry of Education,Ningxia,750001,China)

机构地区：[1]宁夏医科大学宁夏回药现代化工程技术研究中心,宁夏750001 [2]宁夏医科大学宁夏回医药现代化省部共建教育部重点实验室,宁夏750001

出　　处：《亚太传统医药》2020年第5期62-65,共4页Asia-Pacific Traditional Medicine

基　　金：宁夏高等学校科学研究项目(NGY2014105)。

摘　　要：目的:建立超高效液相色谱法同时测定铁棒锤药材中苯甲酰乌头碱、苯甲酰次乌头碱、苯甲酰新乌头碱、乌头碱、次乌头碱、新乌头碱6种生物碱类成分的含量测定方法。方法:采用ACQUITY UPLC BEH C18色谱柱(2.1mm×50mm,1.7μm);乙腈-0.2%冰醋酸水溶液(三乙胺调节PH=6.2)梯度洗脱为流动相;流速0.4mL·min-1;柱温为35℃;检测波长235nm。结果:6种单酯型和双酯型生物碱的线性范围、相关系数良好(r=0.999 4~0.999 9),加样回收率试验的RSD均小于2.0%。结论:该方法简便、快捷、灵敏度高,可用于铁棒锤药材的质量控制。Objective:To establish the UPLC method for simultaneous determination of six esteric alkaloids components benzoylaconine,benzoylhypaconine,benzoylmesaconin,aconitine,hypaconitine,mesaconitine of Aconitum Flaram Hand.Mazz.Methods:ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7μm)was used.The mobile elution,the flow rate was 0.4 mL·min-1.The column temperature was set at 35℃,and the detecting wave length was at 235 nm.RESULTS:The six esteric alkaloids showed good linear relationship(r=0.9994~0.9999),the average recoveries with RSD were less than 2.0%.Conclusion:This method is convenient,quickly,high sensitivity and can be used in the quality control of Aconitum Flaram Hand.Mazz.

关 键 词：铁棒锤 酯型生物碱类 超高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]