SLC2A2基因多态性与2型糖尿病患者二甲双胍疗效的相关性  

Correlation between SLC2A2 gene polymorphism and metformin efficacy in type 2 diabetes mellitus

作　　者：刘阳[1] 杨静[1] 阴津华[1] Liu Yang;Yang Jing;Yin Jinhua(Department of Endocrinology,First Hospital,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学第一医院内分泌科,太原030001

出　　处：《中华内分泌外科杂志》2020年第3期223-227,共5页Chinese Journal of Endocrine Surgery

基　　金：山西省科技成果转化引导专项项目(201804D131044);山西省留学回国人员科技活动择优资助项目(晋人社厅函[2017]397号)。

摘　　要：目的探讨2型糖尿病(T2DM)患者SLC2A2 rs5393、rs5400位点基因多态性与二甲双胍疗效的相关性。方法选取2016年1月至2019年4月期间在山西医科大学第一医院就诊的T2DM患者90例(T2DM组)及90例体检健康者(对照组)作为研究对象,通过PCR-限制性片段长度多态性(PCR-RELP)法检测SLC2A2 rs5393、rs5400位点基因多态性分布频率。对T2DM患者进行二甲双胍治疗,随访90 d,分析二甲双胍疗效与各基因型的关系。结果对照组与T2DM组SLC2A2 rs5393、rs5400各基因型符合Hardy-Weinberg平衡定律。与对照组相比,T2DM组rs5393位点AC型、CC型比例显著升高(P<0.05),rs5400位点CT、TT型频率显著升高(P<0.05)。rs5393位点中,治疗前AA型、AC型、CC型空腹血糖(FBG)、餐后2 h血糖(2 h PBG)、糖化血红蛋白(HbA1c)水平差异无统计学意义(P>0.05);二甲双胍治疗后AC型、CC型患者FPG、2 h PBG、HbA1c降低幅度低于AA型(P<0.05),CC型FPG、HbA1c降低幅度低于AC型(P<0.05)。rs5400位点中,治疗前CC型、CT型、TT型FPG、2 h PBG、HbA1c水平差异无统计学意义(P>0.05);二甲双胍治疗后CT型、TT型患者FPG、2 h PBG、HbA1c降低幅度低于CC型(P<0.05),TT型FPG、HbA1c降低幅度低于CT型(P<0.05)。结论SLC2A2基因多态性位点rs5393 AA型患者、rs5400 CC型T2DM患者对二甲双胍敏感,降糖疗效较好。Objective To investigate the relationship between SLC2A2 rs5393,rs5400 loci gene polymorphism and metformin efficacy in patients with type 2 diabetes mellitus(T2DM).Methods From Jan.2016 to Apr.2019,90 T2DM patients and 90 healthy persons were selected as the subjects.The frequencies of SLC2A2 rs5393 and rs5400 loci gene polymorphisms were detected by PCR restriction fragment length polymorphism(PCR-RELP)method.Patients with T2DM were treated with metformin and followed up for 90 days.The relationship between metformin efficacy and genotypes was analyzed.Results The genotypes of SLC2A2 rs5393 and rs5400 in the control group and T2DM group were in accordance with Hardy Weinberg equilibrium law.Compared with the control group,the ratios of AC type and CC type at rs5393 locus in T2DM group was significantly higher(P<0.05),and the frequencies of CT type and TT type at rs5400 locus were significantly higher(P<0.05).There was no significant difference in the levels of FPG,2 h PBG and HbA1c of AA type,AC type and CC type at rs5393 locus before treatment(P>0.05),after metformin treatment,FPG,2hPBG and HbA1c of patients with AC type and CC type were lower than those with AA type(P<0.05),and the decreases of FPG and HbA1c in CC type were lower than those in AC type(P<0.05).There was no significant difference in the levels of FPG,2hPBG and HbA1c of CC type,CT type and TT type at rs5400 locus before treatment(P>0.05).After metformin treatment,FPG,2 h PBG and HbA1c of patients with CT type and TT type were lower than those with CC type(P<0.05),and the decreases of FPG and HbA1c in TT type were lower than those in CT type(P<0.05).Conclusion T2DM patients with AA type at rs5393 locus and CC type at rs5400 locus of SLC2A2 gene polymorphism loci are sensitive to metformin and has good hypoglycemic effect.

关 键 词：2型糖尿病 SLC2A2基因多态性 二甲双胍 

分 类 号：R58[医药卫生—内分泌] R587.1[医药卫生—内科学]