Macamide B对缺氧缺血性脑损伤新生小鼠脑中雌激素受体表达的影响  

Effects of Macamide B on the expression of estrogen receptor in brain of neonatal mice with hypoxic-ischemic brain damage

作　　者：王朦夏[1] 邢锐[1] 宋云峰[1] 胡英芳[1] 罗文娟[1] 王灿敏[1] Wang Mengxia;Xing Rui;Song Yunfeng;Hu Yingfang;Luo Wenjuan;Wang Canmin(Intensive Care Unit,Guangdong No.2 Provincial People's Hospital,Guangzhou 510317,China)

机构地区：[1]广东省第二人民医院重症医学科,广州510317

出　　处：《神经解剖学杂志》2020年第3期295-300,共6页Chinese Journal of Neuroanatomy

基　　金：广东省中医药局科研课题面上项目(20191015);广东省医学科学基金(A20200252);广东省第二人民医院院内青年基金(YQ2017-001)。

摘　　要：目的:观察玛卡酰胺B(Macamide B)对缺氧缺血性脑损伤(HIBD)新生小鼠脑组织中雌激素受体α(ERα)和雌激素受体β(ERβ)表达的影响.方法:选择出生7 d C57BL/6小鼠30只并将其分为3组:假手术组(sham)、缺氧缺血性脑损伤组(HIBD)、玛卡酰胺B处理组(Macamide B+HIBD),每组10只.采用Rice法建立新生小鼠HIBD模型,采用免疫荧光组织化学染色对ERα和ERβ在新生小鼠HIBD脑内的细胞定位进行分析,Western Blot方法检测Macamide B对新生鼠HIBD脑内ERα和ERβ表达的影响.结果:ERα和ERβ在HIBD新生小鼠脑内神经元和星形胶质细胞中均有表达;与sham组相比,HIBD组ERα和ERβ的表达量均明显降低;与HIBD组相比,Macamide B可显著上调ERα和ERβ的表达.结论:Macamide B可通过上调新生小鼠脑内ERα和ERβ表达表达水平对HIBD发挥神经保护作用.Objective: To observe the effects of macamide B on the expression of estrogen receptor α(ER α) and estrogen receptor β(ER β) in the brain of neonatal mice with hypoxic-ischemic brain damage(HIBD),and investigate the neuroprotective effect of Macamide B on HIBD newborn mice.Methods: Thirty C57BL/6 mouse pups at postnatal 7 day were used and divided into the following three groups: sham group(sham),HIBD group(HIBD),and HIBD pretreated with Macamide B group(Macamide B + HIBD).The HIBD model was established using a modified Rice method.Immunofluorescence histochemical staining was used to analyze the cellular localization of ER α and ER β in the brain of neonatal mice with HIBD;The effect of Macamide B on the expression of ER α and ER β in the brain of neonatal mice with HIBD was detected by Western Blot.Results: ER α and ER β were expressed in both neurons and astrocytes.The expression levels of ER α and ER β in HIBD group were significantly lower than those in sham group.Compared with the HIBD group,the expression levels of ERα and ER β in the Macamide B + HIBD group were significantly increased,and the difference was statistically significant.Conclusion: The neuroprotective effect of Macamide B on neonatal mouse HIBD may be related to the up-regulation of ER α and ER β expression in the brain.

关 键 词：玛卡酰胺B 缺氧缺血性脑损伤 雌激素受体Α 雌激素受体Β 小鼠 

分 类 号：R722.1[医药卫生—儿科]