淫羊藿素对儿童复发急性淋巴细胞白血病细胞的抗白血病效应及其机制  

Anti-leukemia effect of icaritin and its mechanisms on cell line of children relapsed acute lymphoblastic leukemia

作　　者：旷文勇[1] 郑敏翠[1] 李婉丽[1] 杨海霞[1] 李睿娟[2] Kuang Wenyong;Zheng Mincui;Li Wanli;Yang Haixia;Li Ruijuan(Department of Hematology,Hunan Children′s Hospital,Changsha 410007,China;Department of Hematology,the Second Xiangya Hospital of Central South University,Changsha 410011,China)

机构地区：[1]湖南省儿童医院血液内科,长沙410007 [2]中南大学湘雅二医院血液科,长沙410011

出　　处：《中国医师杂志》2020年第6期866-870,874,共6页Journal of Chinese Physician

基　　金：湖南省中医药局一般项目(201670)。

摘　　要：目的:研究淫羊藿素(Icaritin)对儿童复发急性淋巴细胞白血病697细胞的抗白血病效应,并探讨其可能的作用机制。方法:体外培养697细胞,分为对照组和淫羊藿素组(5、10、20μmol/L)。采用CCK8法检测淫羊藿素对697细胞的增殖抑制作用,采用Hochest33258染色和流式细胞检测法检测淫羊藿素对697细胞的凋亡诱导作用;应用Western blot检测Bcl-2、Bax、Cyt-C和Caspase3蛋白的表达。结果:淫羊藿素在5~20μmol/L浓度范围内作用24~72 h对697细胞有增殖抑制作用,这种作用呈剂量和时间依赖关系(r=0.76,r=0.89);淫羊藿素在5~20μmol/L浓度范围内作用48 h对697细胞有凋亡诱导作用(P<0.05);10、20μmol/L淫羊藿素作用697细胞48 h后,697细胞Bcl-2蛋白的表达显著减少(P<0.05),而Bax,Cyt-C和Caspase3裂解片段等蛋白表达显著增高(P<0.05)。结论:淫羊藿素可通过抑制697细胞增殖及诱导其凋亡而发挥抗白血病效应,其机制可能通过上调促凋亡蛋白Bax、Cyt-C和下调抗凋亡Bcl-2而诱导697细胞凋亡。Objective To investigate the anti-leukemia effect of icaritin on 697 cells of children relapsed acute lymphoblastic leukemia in vitro,and to analyze its possible mechanism.Methods 697 cells were cultured in vitro and divided into control group and icariin groups(5,10,20μmol/L).After treated with icaritin,the cell proliferation was detected with cell counting kit-8(CCK-8)method,and apoptosis was measured with Hoechst 33258 staining and Flow Cytometry Assay.Expression of Bcl-2,Cyt-C,Bax and Caspase3 proteins in 697 cell were detected by Western blot.Results After treatment with various concentrations(5-20μmol/L)of icaritin for different time-points(24,48,72 h),the growth inhibition rate exhibited a dose and time-dependent manner(r=0.76,r=0.89);the apoptotic rate exhibited significant increase for 48 h(P<0.05).Bcl-2 protein decreased significantly(P<0.05),while Bax,Cyt-C and Caspase3 cleavage protein increased significantly in icaritin treated group for 48 h compared with control group(P<0.05).Conclusions Icaritin shows the effects of anti-leukemia by inducing apoptosis and inhibiting proliferation of 697 cell.Icaritin maybe induce apoptosis of 697 cell by down-regulation of Bcl-2 expression and up-regulation of the Bax,Cyt-C and cleaved Caspase 3 expression.

关 键 词：淫羊藿甙 急性淋巴细胞性白血病 细胞系 肿瘤 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]