Hydroxycoumarin efficiently inhibits spring viraemia of carp virus infection in vitro and in vivo  

作　　者：Lei Liu Da-Wei Song Guang-Lu Liu Li-Peng Shan Tian-Xiu Qiu Jiong Chen 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Ningbo University,Ningbo,Zhejiang 315211,China [2]Laboratory of Biochemistry and Molecular Biology,School of Marine Sciences,Meishan Campus,Ningbo University,Ningbo,Zhejiang 315832,China [3]Key Laboratory of Applied Marine Biotechnology of Ministry of Education,Meishan Campus,Ningbo University,Ningbo,Zhejiang 315832,China [4]School of Chemistry&Chemical Engineering,Zhoukou Normal University,Zhoukou,Henan 466001,China

出　　处：《Zoological Research》2020年第4期395-409,共15页动物学研究（英文）

基　　金：This work was supported by the Program for the National Natural Science Foundation of China(31902410);Natural Science Foundation of Ningbo City of China(2019A610419);General Scientific Research Project of Education of Zhejiang Province(Y201839187);Scientific Innovation Team Project of Ningbo(2015C110018);Scientific Research Fund of Ningbo University(XYL19017);K.C.Wong Magna Fund in Ningbo University。

摘　　要：Spring viremia of carp virus(SVCV)causes devastating losses in aquaculture.Coumarin has an advantageous structure for the design of novel antiviral agents with high affinity and specificity.In this study,we evaluated a hydroxycoumarin medicine,i.e.,7-(6-benzimidazole)coumarin(C10),regarding its anti-SVCV effects in vitro and in vivo.Results showed that up to 12.5 mg/L C10 significantly inhibited SVCV replication in the epithelioma papulosum cyprini(EPC)cell line,with a maximum inhibitory rate of>97%.Furthermore,C10 significantly reduced cell death and relieved cellular morphological damage in SVCV-infected cells.Decreased mitochondrial membrane potential(ΔΨm)also suggested that C10 not only protected mitochondria,but also reduced apoptosis in SVCVinfected cells.For in vivo studies,intraperitoneal injection of C10 resulted in an anti-SVCV effect and substantially enhanced the survival rate of virusinfected zebrafish.Furthermore,C10 significantly enhanced antioxidant enzyme activities and decreased reactive oxygen species(ROS)to maintain antioxidant-oxidant balance within the host,thereby contributing to inhibition of SVCV replication.The up-regulation of six interferon(IFN)-related genes also demonstrated that C10 indirectly activated IFNs for the clearance of SVCV in zebrafish.This was beneficial for the continuous maintenance of antiviral effects because of the low viral loads in fish.Thus,C10 is suggested as a therapeutic agent with great potential against SVCV infection in aquaculture.

关 键 词：VIVO thereby MAINTAIN 

分 类 号：S941.41[农业科学—水产养殖]