中国樟树初级核心种质取样方法与策略研究  

Sampling Strategy of Primary Core Germplasm of Cinnamomum camphora in China

作　　者：钟永达[1,2] 刘立盘 李彦强[1] 吴照祥[1] 杨爱红 刘淑娟[1] 刘巧丽[1] 刘腾云 余发新[1] Zhong Yongda;Liu Lipan;Li Yanqiang;Wu Zhaoxiang;Yang Aihong;Liu Shujuan;Liu Qiaoli;Liu Tengyun;Yu Faxin(The Key Laboratory of Horticultural Plant Genetic and Improvement of Jiangxi/Institute of Biological Resources,Jiangxi Academy of Sciences,Nanchang Jiangxi 330096,China;Collaborative Innovation Center of Jiangxi Typical Trees Cultivation and Utilization,Nanchang Jiangxi 330045,China)

机构地区：[1]江西省科学院生物资源研究所/江西省观赏植物遗传改良重点实验室,江西南昌330096 [2]江西特色林木资源培育与利用2011协同创新中心,江西南昌330045

出　　处：《西南林业大学学报（自然科学）》2020年第4期1-13,共13页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31860079)资助;江西省林业科技创新专项(201701)资助。

摘　　要：为了优化樟树种质资源保存策略,提高利用效率,利用872份中国樟树种质资源的苗高、地径、种子大小和千粒质量4个表型数据为材料,从分组方法、组内抽样比例方法、聚类方法、抽样策略、总体取样比例等方面进行中国樟树初级核心种质取样策略研究。结果表明:不同聚类方法和取样策略下采用4种系统取样法构建的核心种质的效果是不同的,在确定最长距离法和优先取样策略的前提下,组内采用对数比例法所构建的樟树核心种质效果较好。应用最长距离法进行聚类筛选出的核心种质的极差符合率和变异系数变化率相对高于其他6种方法,是构建樟树核心种质的最佳聚类方法;优先取样法构建的核心种质的遗传多样性指数、表型频率方差、表型方差和变异系数变化率的值都是最佳的。在25%的取样比例下,遗传多样性指数、表型保留比例和表型方差的值最高,表型频率方差最小,是构建樟树初级核心种质的最佳取样比例。综合6个参数的分析结果,最终确定种源-对数比例-最长距离法聚类-优先取样法为构建中国樟树核心种质的优选取样策略。In order to optimize the conservation strategy of camphor tree germplasm resources and improve the utilization efficiency,we used 4 phenotypic data of 872 camphor trees in China,including seedling height,ground diameter,seed size and weight,to research the primary core germplasm sampling strategy of camphor tree in China in this study,from the aspects including grouping method,intra-group sampling proportional method,clustering method,sampling strategy,and overall sampling ratio.The results showed that the effect of the core germplasm constructed was different by 4 kinds of systematic sampling methods under different clustering methods and sampling strategies.Under the premise of determining the longest distance method and the preferential sampling strategy,the core germplasm construction of camphor tree was better by the logarithmic proportion method in the group.The range coincidence rate and coefficient of variation rate of the core germplasm screened by the longest distance method were higher than those of the other 6 methods,which was the best clustering method.The values of genetic diversity index,phenotypic frequency variance,phenotypic variance and the variable rate of coefficient of variation of core germplasm constructed by the preferential sampling method were all the best.The genetic diversity index,phenotypic retention ratio and phenotypic variance were the highest and the phenotypic frequency variance was the lowest at 25%sampling ratio,indicating which was the best sampling ratio to construct the primary core germplasm of camphor tree.Based on the analysis results of 6 parameters,the provenance-logarithmic proportion-longest distance clustering-preferential sampling method is finally determined as the optimal sampling strategy for the construction of core germplasm of camphor tree in China.

关 键 词：樟树 种质资源 核心种质 取样策略 

分 类 号：S792.23[农业科学—林木遗传育种]