日本血吸虫感染对鸡卵清蛋白诱导的小鼠过敏性气道炎症的影响  

Effects of Schistosoma japonicum infection on OVA-induced allergic airway inflammation in mice

作　　者：李志丹 张卫 王晓玲 徐斌 胡薇 LI Zhi-dan;ZHANG Wei;WANG Xiao-ling;XU Bin;HU Wei(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research,WHO Collaborating Centre for Tropical Diseases\National Center for International Research on Tropical Diseasesy Ministry of Science and Technology\Key Laboratory of Parasite and Vector Biology,Ministry of Health,Shanghai 200025,China;School of Life Sciencesy Fudan University,Shanghai 200025,China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,国家热带病研究中心,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室,上海200025 [2]复旦大学,生命科学学院,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2020年第3期271-278,共8页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家基础科研项目(No.YFA20180507300)。

摘　　要：目的研究日本血吸虫感染(感染后21 d)对鸡卵清蛋白(OVA)诱导的小鼠过敏性气道炎症的影响。方法20只雌性BALB/c小鼠随机分为健康对照组、感染组、OVA组、感染+OVA组,每组5只。感染组和感染+OVA组每鼠经腹部贴片法感染日本血吸虫尾蚴(15±2)条。感染后第21、35天,感染+OVA组和OVA组接受腹腔注射10μg OVA+2 mg铝佐剂致敏,感染后第42~46天接受1%OVA(PBS溶液)雾化激发,30 min/次,每天1次;健康对照组和感染组小鼠给予PBS雾化激发。采集感染后第35天(致敏期)、42天(致敏期)、49天(激发期)各组小鼠尾静脉血,ELISA检测血清IgE抗体和OVA特异性IgE抗体水平。感染后第49天剖杀全部小鼠,取肺和脾组织,取部分肺组织制作病理切片,苏木精伊红(HE)和过碘酸希夫氏(PAS)染色后观察肺组织血管、支气管周围炎症(炎症指数)和上皮细胞增生(增生指数)的病理学变化;另取一部分肺组织和脾组织制备单细胞悬液,流式细胞术检测CD4^+CD25^+Foxp3^+调节性T(Treg)细胞的比例。结果ELISA检测结果显示,感染后第35、42和49天,感染组小鼠血清IgE抗体的吸光度(A450值)分别为0.291±0.037、0.388±0.038和0.472±0.053,均高于健康对照组[(0.032±0.018、0.050±0.016和0.043±0.026)](P<0.01);OVA组分别为0.097±0.065、0.164±0.083和0.274±0.073,第42天和49天IgE抗体水平较健康对照组升高(P<0.01);感染+OVA组分别为0.305±0.054、0.359±0.037和0.454±0.017,均高于OVA组(P<0.01)。感染后第35、42和49天,OVA组OVA特异性IgE抗体的A450值分别为0.115±0.021、0.078±0.014和0.245±0.040,均高于健康对照组[(0.081±0.007、0.054±0.008和0.079±0.008)](P<0.01、0.05、0.05);感染+OVA组分别为0.034±0.009、0.038±0.016和0.203±0.017,均低于OVA组(P<0.01、0.01、0.05)。感染后第49天的病理学检测结果显示,健康对照组、感染组、OVA组和感染+OVA组小鼠肺组织支气管和血管周围炎细胞浸润的炎症指数分别为1.33±0.47、2Objective To investigate the effects of Schistosoma japonicum infection(21 days after infection)on ovalbumin(OVA)-induced allergic airway inflammation in mice.Methods Twenty female BALB/c mice were randomly divided into the healthy control group,infection group(INF),OVA group and infection+OVA group(INF+OVA),5 mice each.Mice of the INF and INF+OVA groups were infected with 15±2 S.japonicum cercariae by abdomen patches.Mice in the INF+OVA group and OVA group were intraperitoneally injected with 10μg of OVA plus 2 mg of aluminum adjuvant to sensitize on day 21 and 35 after infection,and were given with aerosolized 1%OVA in PBS/mouse to challenge on day 42-46 after infection,whereas the mice of healthy control and infection group were challenged with PBS aerosol.Mice tail vein blood was collected on day 35 and 42(sensitizing phase),and day 49(challenging phase)for determination of serum IgE and OVA-specific IgE by ELISA.All mice were sacrificed on day 49 after infection,and lung and spleen tissues were collected.A part of the lung tissue was used to prepare pathological paraffin sections stained by hematoxylin eosin(HE)and periodic acid Schiff(PAS),to observe morphological changes in lung tissue vessel,inflammation peri-bronchi(inflammation score),and in hyperplasia of epithelial cells(hyperplasia score);the other part of lung and spleen tissues was used to prepare single cell suspensions for assaying proportions of CD4^+CD25^+Foxp3^+Tregs by flow cytometry.Results ELISA showed that the A450 readings of serum IgE in the INF group on day 35,42 and 49 were 0.291±0.037,0.388±0.038 and 0.472±0.053,respectively,which were significantly higher than those in healthy control(0.032±0.018,0.050±0.016 and 0.043±0.026)(all P<0.01);the A450 readings of IgE in the OVA group on day 35,42 and 49 were 0.097±0.065,0.164±0.083 and 0.274±0.07,respectively,of which the readings on day 42 and 49 were significantly higher than those in healthy control(P<0.01);the A450 readings of IgE in the INF+OVA group on those days were 0.30

关 键 词：日本血吸虫 过敏性气道炎症 鸡卵清蛋白 调节性T细胞 IGE抗体 

分 类 号：R383.24[医药卫生—医学寄生虫学]