隐孢子虫体外培养模型研究进展  

Research progress on the in vitro culture model of Cryptosporidium species

作　　者：牛子文 常艳凯 王克 张龙现 NIU Zi-wen;CHANG Yan-kai;WANG Ke;ZHANG Long-xian(College of Animal Science and Veterinary Medicine,Henan Agricultural University,International Joint Research Laboratory for Zoonotic Diseases of Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南农业大学牧医工程学院,河南省人兽共患病国际联合实验室,郑州450046

出　　处：《中国寄生虫学与寄生虫病杂志》2020年第3期394-399,共6页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：科技部重点研发计划项目(No.2017YFD0500400);国家自然科学基金(No.31672548)。

摘　　要：隐孢子虫是一种重要的人兽共患寄生原虫,主要寄生于肠上皮细胞并可引起人和动物腹泻。目前尚无有效针对隐孢子虫病的防控药物和疫苗。建立高效培养模型可为研究隐孢子虫入侵宿主细胞机制、抗隐孢子虫疫苗和药物的研发提供试验基础。与常见的动物感染模型相比,体外培养系统成本较低,可以模拟宿主生理状况,便于筛选抗隐孢子虫药物。本文对隐孢子虫鸡胚及鸡胚器官培养模型、细胞培养模型、无细胞培养模型、三维培养模型以及肠道类器官培养模型的研究进展进行综述,为深入了解隐孢子虫致病机制及抗隐孢子虫的药物研发提供参考。Cryptosporidium is an important zoonotic protozoan parasite,which mainly parasitizes in intestinal epithelial cells,causing diarrhea in humans and animals.To date,there are no effective drugs or vaccines for control of cryptosporidiosis.Establishing an efficient culture model may provide experimental basis for studying the mechanisms of host cell invasion by Cryptosporidium and the development of vaccines and drug against Cryptosporidium.Comparing to common animal infection models,in vitro culture system has advantages of low cost and being able to simulate physiological status of hosts,compared to common animal models of infection,thus facilitating anti-Cryptosporidium drug screen.This article reviews the research progress on chicken embryo and chicken embryo organ culture models,the cell culture models,the cell-free culture model,the three-dimensional culture model and intestinal organoids culture model for Cryptosporidium,to provide reference for further understanding of pathogenic mechanisms of Cryptosporidium and the development of anti-Cryptosporidium drugs.

关 键 词：隐孢子虫 体外培养模型 研究进展 

分 类 号：R382.9[医药卫生—医学寄生虫学]