酿酒废水硫氧化细菌的分离鉴定及其硫氧化特性  

Isolation and Identification of Sulfur-oxidizing Bacterium from Brewery Wastewater and Its Sulfur-oxidizing Characteristics

作　　者：韩佳悯 罗剑飞[1] 林炜铁[1] HAN Jia-min;LUO Jian-fei;LIN Wei-tie(School of Biology and Biological Engineering,South China University of Technology,Guangzhou 510006,China)

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006

出　　处：《现代食品科技》2020年第6期155-160,共6页Modern Food Science and Technology

摘　　要：本研究从酿酒废水中分离了一株化能自养硫氧化细菌LS2,通过16S rRNA基因和硫氧化功能酶基因sox B分析表明该菌株与Halothiobacillus亲缘关系最近,但Halothiobacillussp.LS2的基因组中存在完整的固氮酶基因簇nif,其关键编码基因nif H与Acidithiobacillus属中A. ferrooxidans和A. ferrivorans相似性最高,均高于80%。通过细胞水平的比较研究,菌株LS2在有氮和无氮条件下均可生长,在1 mM NH4^+的条件下生长量最高可达1.98×10^7/m L,而无氮条件下生长量较有氮条件下少,最高可达1.37×10^7/m L;基于基因表达分析,菌株LS2的nif H表达量因氮源存在而下调,硫代硫酸盐浓度增加而上调,其中在5 mM S2O32-浓度下其表达量上调了约33倍。说明该菌的固氮作用与硫氧化反应息息相关。本研究获得了一株具有固氮能力的化能自养硫氧化细菌,所获得的结果可为含硫化物废水的治理提供一种新思路。In this study,a chemoautotrophic sulfur-oxidizing bacterial strain LS2 was isolated from brewery wastewater.The analyses of 16S rRNA gene and sulfur-oxidizing enzyme gene soxB revealed that this strain was most closely related to genus Halothiobacillus,although the genome of Halothiobacillus sp.LS2 had a complete gene cluster encoding nitrogen-fixing enzyme Nif;Its key gene nifH had the highest similarity to A.ferrooxidans and A.ferrivorans in the genus Acidithiobacillus(both of which were higher than 80%).A comparative study at the cellular level indicated that the strain LS2 was able to grow under both nitrogen and nitrogen-free conditions.The maximum growth of LS2 under the condition of 1 mM NH4^+could reach 1.98×10^7/mL,whilst the growth in the absence of nitrogen(up to 1.37×10^7/mL)was less than that in the presence of nitrogen.The gene expression analysis revealed that nifH expression of strain LS2 was down-regulated due to the presence of a nitrogen source but up-regulated with the increase of thiosulfate concentration.The expression of nifH was up-regulated by 33 times at a S2O32-concentration of 5 mM,indicating that nitrogen fixation was closely related to sulfur oxidation.In this study,a chemoautotrophic sulfur-oxidizing bacterium with nitrogen-fixing ability was obtained,and the obtained results can provide a new idea for the treatment of sulfide-containing wastewater.

关 键 词：化能自养型细菌 硫氧化作用 固氮作用 

分 类 号：X797[环境科学与工程—环境工程]