液质联用中接口离子化新技术  

New Ionization Technology for Interface of Liquid Chromatography-Mass Spectrometry

作　　者：田甜[1] 张芳[1] 张曙盛 冯陈国 苏越 林国强 Tian Tian;Fang Zhang;Shusheng Zhang;Chenguo Feng;Yue Su;Guoqiang Lin(TheResearch Center of Chiral Drugs,InnovationResearch Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Center for Chinese Medicine Therappy and Systems Biology,Institute for Interdisciplinary Medicine Sciences,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Key Laboratory of Synthetic Chemistry of Natural Substances,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032,China)

机构地区：[1]上海中医药大学创新中药研究院手性药物研究中心,上海201203 [2]上海中医药大学交叉科学研究院中医方证与系统生物学研究中心,上海201203 [3]中国科学院上海有机化学研究所天然产物有机化学重点实验室,上海200032

出　　处：《化学进展》2020年第5期604-616,共13页Progress in Chemistry

基　　金：国家自然科学基金项目(No.21672249)资助。

摘　　要：液相色谱-质谱联用(简称液质联用,LC-MS)将色谱的高分离效能与质谱强大的结构测定功能结合,不仅实现了对复杂混合物更准确的定性定量分析,而且简化了样品的前处理过程,使样品分析更简便,在药物分析、食品与环境分析以及生物样品检测等众多领域得到了广泛的应用。作为LC-MS的核心组成部分,液质接口的作用是将LC的液体引入,发生电离,并将生成的离子传输进MS。因此,接口离子化技术的改进直接影响了LC-MS的发展和应用。为了获得更高的灵敏度和更广泛的适用性,研究人员一直致力于离子化技术的研究,以促进分析物的解吸,提高其电离和传输效率,减少基质效应的干扰。本文针对近年来LC-MS接口离子化技术的改进和发展,从离子化原理出发,对接口离子源的构造、影响电离的因素、以及相关的应用进行综述,探讨其优缺点,并对LC-MS接口离子化技术的发展趋势进行了展望。Liquid chromatography-mass spectrometry(LC-MS) combines the high separation efficiency of chromatography with the powerful structural determination of mass spectrometry, which not only enables more accurate analysis for the compounds, but also simplifies the pre-treatment of samples and makes the analysis more convenient. LC-MS, as an important tool for the qualitative and quantitative analysis of organic compounds, has been widely used in various fields such as pharmaceutical analysis, food and environmental monitoring, biological and medical research etc. As the key component of LC-MS, the role of the interface is to introduce and ionize the fractions from LC, and transfer the generated ions into the MS. Therefore, the improvement of ionization technology for the interface directly affects the advance and application of LC-MS. In order to obtain a higher sensitivity and a wider range of applicability, researchers have focused on the ionization technology to promote the desorption of chemicals, improve their ionization and transport efficiency, and reduce the interference of matrix effect. In this work, the development of traditional ionization technologies and the novel technologies reported for the interface of LC-MS in recent years are reviewed, including the ionization principle, interface construction, influencing factors, and the related applications. Their characteristics, advantages and disadvantages are discussed in details. Finally, the trend of ionization technology in development for the interface of LC-MS is prospected.

关 键 词：液质联用 接口 离子化 

分 类 号：O657.63[理学—分析化学]