正己烷亚急性染毒对小鼠运动能力的影响及其机制研究  

Effects of n-hexane on the movement ability and its mechanisms in mouse with sub-acute exposure

作　　者：赵文恒 张天昊 王文龙 王宝强 苏艳霞 林立[1] ZHAO Wenheng;ZHANG Tianhao;WANG Wenlong;WANG Baoqiang;SU Yanxia;LIN Li(School of Public Health,Jining Medical University,Jining 272013,China;School of Public Health,Qingdao University,Qingdao 266071,China)

机构地区：[1]济宁医学院公共卫生学院,济宁272013 [2]青岛大学公共卫生学院,青岛266071

出　　处：《济宁医学院学报》2020年第3期153-156,共4页Journal of Jining Medical University

基　　金：国家级大学生创新项目(201810443038)。

摘　　要：目的 研究正己烷亚急性染毒对小鼠运动能力的影响及其生化机制。方法 将小鼠随机分为对照组和低、中、高剂量染毒组,每组20只。低、中、高剂量染毒组分别按500 mg/kg、1000 mg/kg、2000 mg/kg的剂量进行正己烷灌胃染毒,每天1次,共28d。染毒结束后,对各组小鼠进行负重游泳实验,并进行血清葡萄糖、尿素氮以及骨骼肌ATP酶、脂质过氧化指标的测定。结果 低、中、高剂量染毒组负重游泳时间分别为(57. 20±14. 17)、(9. 02±2. 23)、(5. 85±2. 94) min,血糖浓度分别为(13. 88±1. 76)、(12. 52±1. 51)、(10. 96±2. 20)mmol/L;血清尿素氮浓度分别为(12. 34±1. 76)、(17. 64±3. 52)、(23. 02±2. 58) mmol/L,骨骼肌Na+-K+-ATP酶活力分别为(12. 29±2. 77)、(7. 05±1. 45)、(4. 75±0. 72)μmol Pi/gprot/h,Ca2+-Mg2+-ATP酶的活力分别为(1. 41±0. 35)、(0. 41±0. 12)、(0. 39±0. 09)μmol Pi/gprot/h;骨骼肌MDA浓度分别为(4. 77±1. 05)、(6. 62±1. 84)、(6. 98±2. 22)μmol/mgprot;SOD活力分别为(75. 45±19. 43)、(88. 37±24. 10)、(105. 63±31. 09) U/mgprot;GSH-Px活力分别为(66. 25±21. 54)、(79. 30±26. 97)、(91. 85±32. 58) U/mgprot。与对照组比较,3个染毒组游泳时间明显缩短,高剂量染毒组、中剂量染毒组血清尿素氮浓度明显升高(P <0. 01);除低剂量染毒组的Na+-K+-ATP酶活力外,高剂量染毒组和中剂量染毒组Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶的活力显著下降(P<0. 01);除低剂量染毒组的MDA浓度外,3个染毒组各项脂质过氧化指标均明显升高(P <0. 01)。结论 正己烷亚急性染毒导致小鼠的运动耐力下降,一方面由于血糖水平降低而导致ATP产生和储备下降,另一方面由于脂质过氧化增强所致骨骼肌细胞的损伤而使其对ATP的利用率下降。Objective To study the effects of n-hexane on the movement ability and its biochemical mechanisms in mouse with sub-acute exposure.Methods Mice were randomly divided into control group,lowdose group,middle-dose group and high-dose group.The three dose groups were exposed to n-hexane once a day for 28 days by the doses of 500 mg/kg,1000 mg/kg and 2000 mg/kg respectively.At the end of the exposure,the swimming experiment,the serum concentrations of glucose and urea nitrogen and the ATPase activities and lipid peroxidation indexes of skeletal muscle were measured.Results The swimming times were57.20±14.179.02±2.23 and 5.85±2.94 min;the serum glucose concentrations were 13.88±1.76,12.52±1.51 and 10.96±2.20 mmol/L;the serum urea nitrogen concentrations were 12.34±1.76,17.64±3.52 and23.02±2.58 mol/L;the Na+-K+-ATPase activities of skeletal muscle were 12.29±2.77,7.05±1.45 and 4.75±0.72μmolPi/gprot/h;the Ca2+-Mg2+-ATPase activities of were 1.41±0.35,0.41±0.12 and 0.39±0.09μmolpi/gprot/h;MDA concentrations of skeletal muscle were 4.77±1.05,6.62±1.84 and 6.98±2.22 mmol/gprot;SOD activities were 75.45±19.43,88.37±24.10 and 105.63±31.09 U/mgprot;and GSH Px activities were 66.25±21.54,79.30±26.97 and 91.85±32.58 U/mgprot,respectively.Compared with the control group,the swimming time of the three dose groups was decreased significantly;the serum urea nitrogen concentrations was increased and the serum glucose concentrations was decreased significantly in the high-dose group and the middle-dose group(P<0.01).The Na+-K+-ATP ase and Ca2+-Mg2+-ATPase activities of the high-dose group and the middle-dose group were significantly decreased(P<0.01).The indexes of lipid peroxidation in the three dose groups were significantly increased(P<0.01).Conclusion The subacute exposure of n-hexane in mouse could resulted in the decrease of exercise endurance,and on the one hand the causes of which was due to the decrease of blood glucose level was related to the decease of ATP utilization induced by the enhancement of

关 键 词：正己烷 运动能力 ATP酶 脂质过氧化 小鼠 

分 类 号：R135.1[医药卫生—劳动卫生]