Effects of different transdermal penetration enhancers applied to herbal cake-partitioned moxibustion on liver lipids,HSL and HMG-CoA reductase in hyperlipidemia rabbits  

不同促透剂用于隔药饼灸对高脂血症兔肝脏脂质及HSL和HMG-CoA还原酶的影响

作　　者：Liao Zong-li Zhu Chong-zheng Tan Jing Luo Feng-jiao Sun Lu Huang Wen-tao Chen Yan-ping Yang Ren-da Chang Xiao-rong 廖宗力;朱重政;谭静;罗凤姣;孙璐;黄文韬;陈艳萍;阳仁达;常小荣(Hunan University of Chinese Medicine,Changsha 410208,China;Xiniujiao Community Health Service Station,Jingxi Street,Baiyun District in Guangzhou City,Guangdong 510515,China;Huizhou Hospital of Traditional Chinese Medicine,Guangdong 516000,China;Department of Stomach Surgery,Sun Yat-sen University Cancer Center,Guangdong 510060,China;The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China)

机构地区：[1]Hunan University of Chinese Medicine,Changsha 410208,China [2]Xiniujiao Community Health Service Station,Jingxi Street,Baiyun District in Guangzhou City,Guangdong 510515,China [3]Huizhou Hospital of Traditional Chinese Medicine,Guangdong 516000,China [4]Department of Stomach Surgery,Sun Yat-sen University Cancer Center,Guangdong 510060,China [5]The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China

出　　处：《Journal of Acupuncture and Tuina Science》2020年第3期157-164,共8页针灸推拿医学（英文版）

基　　金：湖南省高校创新平台开放基金项目,No.16K068;2017年湖南省研究生科研创新项目,No.CX2017B431;湖南省大学生创新实验项目,No.2017-132.

摘　　要：Objective:To observe the effects of laurocapram and borneol as transdermal penetration enhancers applied to herbal cake-partitioned moxibustion on liver lipids,hormone-sensitive lipase(HSL)and hydroxymethylglutaryl CoA(HMG-CoA)reductase in hyperlipidemia rabbits.Methods:Forty New-Zealand rabbits were randomly divided into 5 groups using the random number table method,with 8 rats in each group.Rabbits in the blank group were fed routinely with a normal diet;rabbits in the other groups were fed with high-fat diet for 12 weeks to establish the hyperlipidemia model.Rabbits in the blank and the model groups were not given any intervention.After the model was prepared successfully,rabbits in the non-transdermal penetration enhancer group received herbal cake-partitioned moxibustion without transdermal penetration enhancers;rabbits in the laurocapram group and the borneol group received herbal cake-partitioned moxibustion with laurocapram or borneol respectively.After 4 weeks of treatment,the serum was isolated and enzyme-linked immunosorbent assay(ELISA)was applied for the detection of HSL and HMG-CoA reductase.The liver tissues were isolated,and total cholesterol(TC)and triglycerides(TG)were measured by enzymatic methods.One-step method was applied for high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)detection,and transmission turbidimetry was for apolipoprotein A1(Apo-A1)and apolipoprotein B(Apo-B)detection.Results:The serum concentrations of the drugs in the laurocapram and the borneol groups were significantly higher than those in the non-transdermal penetration enhancer group(both P<0.05);all drug penetrations in the borneol group were significantly higher than those in the laurocapram group(both P<0.05),except for tanshinoneⅡA.Compared with the non-transdermal penetration enhancer group,the HSL was significantly increased while the HMG-CoA reductase was significantly decreased in the laurocapram and the borneol groups(both P<0.05);between groups,the HSL in the borneol gro目的:探讨冰片和氮酮作为促透剂用于隔药饼灸对高脂血症兔肝脏脂质及激素敏感脂肪酶(HSL)和羟甲基戊二酰辅酶A(HMG-CoA)还原酶的影响.方法:将40只新西兰兔按随机数字表法随机分成5组,每组8只.空白组正常喂养普通饲料;其余组高脂饲料喂养12周,复制高脂模型.空白组和模型组不治疗.无促透剂组成模后采用不加促透剂的药饼施灸;氮酮组和冰片组分别将氮酮和冰片加于药饼中,进行隔药饼灸.治疗4周后,分离血清,酶联免疫吸附法(ELISA)检测HSL和HMG-CoA还原酶.取肝组织采用酶法测定总胆固醇(TC)和甘油三酯(TG),直接一步法测定高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C),透射比浊法测定载脂蛋白A1(Apo-A1)及载脂蛋白B(Apo-B).结果:氮酮组和冰片组血清药物浓度显著高于无促透剂组(均P<0.05);除丹参酮ⅡA外,冰片组其他药物渗透量均显著高于氮酮组(均P<0.05).氮酮组和冰片组HSL显著高于无促透剂组,HMG-CoA还原酶显著低于无促透剂组(均P<0.05);冰片组HSL显著高于氮酮组(P<0.05).模型组兔肝脏LDL-C、TG、TC和Apo-B含量显著高于空白组(P<0.05);无促透剂组、氮酮组和冰片组LDL-C、TG、TC及Apo-B含量显著低于模型组(均P<0.05),氮酮组TG和TC显著低于无促透剂组(均P<0.05),冰片组LDL-C、TG、TC和Apo-B显著低于无促透剂组(均P<0.05);冰片组TG、LDL-C和Apo-B显著低于氮酮组(均P<0.05);模型组HDL-C和Apo-A1显著低于空白组(均P<0.05),无促透剂组、氮酮组和冰片组HDL-C及Apo-A1显著高于模型组(均P<0.05),氮酮组Apo-A1、冰片组HDL-C及Apo-A1均显著高于无促透剂组(均P<0.05).结论:氮酮和冰片作为促透剂用于隔药饼灸,能促进药饼中药物吸收,降低肝脏TC、TG、LDL-C和Apo-B含量,提高HDL-C和Apo-A1含量,改善HSL和HMG-CoA还原酶的代谢;冰片效果略优于或相当于氮酮.

关 键 词：Moxibustion Therapy Herbal Cake-partitioned Moxibustion HYPERLIPIDEMIAS Laurocapram ISOBORNEOL Sterol Esterase Hydroxymethylglutaryl CoA Reductases RABBITS 

分 类 号：R2-03[医药卫生—中医学]