Effects of Branching Strategy on the Gene Transfection of Highly Branched Poly(β-amino ester)s  

作　　者：Yao Wang Chen-Fei Wang Ming Li De-Zhong Zhou Wei Huang Wen-Xin Wang 

机构地区：[1]School of Chemistry and Chemical Engineering,State Key Laboratory of Metal Matrix Composites,Shanghai Jiao Tong University,Shanghai200240,China [2]School of Chemical Engineering and Technology,Xi'an Jiaotong University,Xi'an 710049,China [3]Department of Dermatology,Xinhua Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China [4]Charles Institute of Dermatology,School of Medicine,University College Dublin,Dublin 4,Ireland [5]School of Mechanical and Materials Engineering,University College Dublin,Dublin 4,Ireland

出　　处：《Chinese Journal of Polymer Science》2020年第8期830-839,I0006,共11页高分子科学（英文版）

基　　金：This work was financially supported by Science Foundation Ireland(SFI)Principal Investigator Program(No.13/IA/1962);the National Natural Science Foundation of China(Nos.51873179,51903202),and University College Dublin。

摘　　要：Highly branched poiy(β-amino ester)s(HPAEs)have emerged as one type of the most viable non-viral gene delivery vectors,both in vitro and in viva.However,the effects of different branching strategies on the gene transfection performance have not yet been explored.Here,using triacrylate(B3)and diamine(B4)as the branching monomers,a series of HPAEs were synthesized via the"A2+B3+C2"and"A2+B4+C2"strategies,respectively.Results show that the branching strategy plays a pivotal role in dictating the physiological properties of the HPAE/DNA polyplexes and thus leads to obviously different cell viability and transfection efficiency.Comparatively,HPAEs synthesized via the"A2+B3+C2"branching strategy are more favorable for DNA transfection than that synthesized via the"A2+B4+C2"strategy.This study may provide new insights into the development of HPAEs based non-viral DNA delivery system.

关 键 词：Gene therapy Non-viral gene vector Highly branched polymer Branching strategy 

分 类 号：TQ317[化学工程—高聚物工业] R450[医药卫生—治疗学]