藻菌共培养对小球藻生长及苯酚降解的影响  

Effect of algae-bacteria co-culture on Chlorella sp.growth and phenol degradation

作　　者：易涛 山鹰 黄渤 唐涛[2] 高旻天 魏伟[2] YI Tao;SHAN Ying;HUANG Bo;TANG Tao;GAO Mintian;WEI Wei(School of Life Sciences,Shanghai University,Shanghai 200444,China;Key Laboratory of Low-Carbon Conversion Science and Engineering,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China)

机构地区：[1]上海大学生命科学学院,上海200444 [2]中国科学院上海高等研究院,低碳转化科学与工程重点实验室,上海201210

出　　处：《环境工程学报》2020年第6期1679-1687,共9页Chinese Journal of Environmental Engineering

摘　　要：工业苯酚废水无序排放会对环境造成极大危害,构建既能去除苯酚又能积累微藻生物质的藻菌组合对实现苯酚废水净化及其资源化利用具有重要意义。首先,研究了小球藻对苯酚的耐受性和降解性能;然后,构建了其与简单芽胞杆菌Bacillus simplex的共培养体系;最后,测试了藻菌比、藻菌接种浓度和苯酚浓度等对小球藻生长及苯酚降解的影响。结果表明:小球藻能耐受400 mg·L-1的苯酚,但其对100~600 mg·L-1苯酚的降解率仅为1.21%~11.66%;对于藻菌共培养体系,在固定小球藻接种浓度为0.2 g·L-1、藻菌比为1∶4~4∶1条件下,3~5 d完全降解了400 mg·L-1的苯酚,小球藻叶绿素(a+b)含量较单藻组增加了0.14~2.21倍,且随着藻菌比降低,苯酚降解效率及小球藻生物量逐步提高;在固定藻菌比为1∶1、小球藻初始接种浓度为0.05~0.4 g·L-1条件下,4~5 d完全降解400 mg·L-1苯酚,且在藻接种浓度为0.2 g·L-1条件下,小球藻具有最高的比生长速率;在藻菌接种浓度0.2 g·L-1、藻菌比1∶1条件下,6 d内完全降解500 mg·L-1的苯酚,且在各苯酚浓度(200~600 mg·L-1)下,小球藻叶绿素(a+b)含量较初始接种值增加了1.54~4.71倍。与简单芽胞杆菌共培养可以促进小球藻生长并提高其苯酚降解能力,在苯酚废水净化及资源化利用领域展现了一定的应用潜力。The discharge of industrial phenol wastewater has caused great harm to the environment. This study aims to construct one microalgae-bacteria consortium for simultaneous phenol removal and microalgae biomass accumulation, which has great significance for purification and resource utilization of phenol wastewater.Firstly, the tolerance and degradation performance of Chlorella sp. to phenol were investigated. Secondly, one consortium was constructed using Chlorella sp. and Bacillus simplex, and then the effects of microalgae to bacteria ratio, microalgae and bacteria inoculation concentrations and initial phenol concentration on phenol degradation and microalgae growth were investigated. The results showed that Chlorella sp. could tolerate 400 mg·L-1 phenol, while it only had degradation rates of 1.21%~11.66% for 100~600 mg·L-1 phenol. For the consortium with Chlorella sp.(0.2 g·L-1) to B. simple ratios ranging from 1∶4 to 4∶1, 400 mg·L-1 phenol was completely degraded within 3~5 d, and the contents of chlorophyll(a+b) increased by 0.14~2.21 times compared to Chlorella sp. Monoculture. The phenol degradation efficiency and microalgae biomass gradually increased with the decrease of Chlorella sp. to B. simplex ratio. For the consortium with fixed Chlorella sp. and B. simplex ratio of 1∶1, and Chlorella sp. inoculation concentrations of 0.05~0.4 g·L-1, 400 mg·L-1 phenol was completely degraded within 4~5 d, and the specific growth rate of Chlorella sp. was highest at the inoculation concentration of 0.2 g·L-1. For the consortium with fixed Chlorella sp. and B. simplex ratio of 1∶1, and Chlorella sp.inoculation concentrations of 0.2 g·L-1, 500 mg·L-1 phenol was completely degraded within 6 d. The content of chlorophyll(a+b) increased by 1.54 to 4.71 times compared to initial inoculation value at phenol concentrations of 200~600 mg·L-1. The above results show that the consortium could significantly improve phenol degradation efficiency and Chlorella sp. growth, and has a certain application potential i

关 键 词：小球藻 藻菌共培养 苯酚降解 高浓度苯酚废水 微藻生物量 

分 类 号：X703[环境科学与工程—环境工程]