穴位埋线对过敏性鼻炎大鼠下气道重塑的影响  被引量:8

Effect of catgut implantation at “Yingxiang”(LI20) on lower airway remodeling in allergic rhinitis rats

在线阅读下载全文

作  者:刘菊华 辜建伟[2] 胡泉[1] 岳国龙[1] 刘磊 涂翔 何成诗[1] 杜继平[3] LIU Ju-hua;GU Jian-wei;HU Quan;YUE Guo-long;LIU Lei;TU Xiang;HE Cheng-shi;DU Ji-ping(Department of Pneumology,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610000,China;Department of Geriatrician,Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province;Department of Pneumology,Shuangliu Hospital of Traditional Chinese Medicine,Chengdu 610000)

机构地区:[1]成都中医药大学附属医院呼吸科,成都610000 [2]川北医学院附属医院老年科,四川南充637000 [3]成都双流区中医院呼吸科,成都610000

出  处:《针刺研究》2020年第6期461-467,共7页Acupuncture Research

基  金:四川省科技计划重点研发项目(No.2017SZ0164);四川省科技厅重大前沿项目(No.2016JY0008)。

摘  要:目的:观察穴位埋线对过敏性鼻炎(AR)大鼠下气道重塑及肺组织骨桥蛋白(OPN)含量的影响,探讨穴位埋线改善AR的部分作用机制。方法:雄性SD大鼠随机分为空白组、模型组及埋线组,每组10只。模型组及埋线组采用腹腔注射联合双侧鼻腔局部滴入卵清蛋白复制AR大鼠模型。埋线组给予双侧"迎香"穴埋线28 d。观察各组大鼠一般症状评分,用苏木精-伊红染色法、过碘酸-雪夫染色法及Masson染色法观察大鼠肺组织形态学改变,用免疫组织化学法及Western blot法检测大鼠肺组织中OPN蛋白表达。结果:治疗前,模型组及埋线组大鼠的一般症状评分均明显高于空白组(P<0.05),提示造模成功;治疗后,埋线组一般症状评分较治疗前明显降低(P<0.05),且明显低于模型组(P<0.05)。空白组大鼠肺组织各级支气管分支结构正常,支气管纤毛上皮排列整齐;模型组大鼠肺组织部分气道管壁增厚、管腔狭窄,管腔内黏液分泌增加,肺泡间隔增宽,支气管周围和肺间质可见炎性细胞浸润;埋线组大鼠肺组织各级支气管分支结构基本正常。与空白组比较,模型组大鼠肺组织中杯状细胞的数量、肺纤维组织吸光度值及OPN蛋白表达水平明显升高(P<0.05);与模型组比较,埋线组大鼠肺组织中杯状细胞的数量及OPN蛋白表达水平明显降低(P<0.05)。肺组织杯状细胞计数和肺纤维组织吸光度值均与OPN蛋白的阳性表达吸光度值、相对表达量成正相关(P<0.05,P<0.01)。结论:穴位埋线治疗可减轻AR大鼠下气道炎性反应,延缓气道重塑,其机制可能与降低肺组织OPN蛋白表达水平相关。Objective To observe the effect of catgut implantation at"Yingxiang"(LI20)on lower airway remodeling and levels of osteopon-tin(OPN)protein in allergic rhinitis(AR)rats,so as to reveal its mechanisms underlying improvement of AR.Methods Male SD rats were randomly divided into control,model and catgut implantation groups,with 10 rats in each group.The AR model was established by intraperitoneal injection and nasal drip of ovalbumin.The catgut implantation was applied to bilateral"Yingxiang"(LI20)for 28 days in rats of the catgut implantation group.The total score of allergic symptoms of rats in each group were observed.The histopathological changes of lower airway were observed under light microscope after Hematoxylineosin,Periodic acid-Schiff and Masson staining.The expression of OPN protein was detected by immunohistochemistry and Western blot,separately.Results The total score of allergic symptoms of nose-wiping,running nose and sneezing,count of lung goblet cells,lung fiber content,and immunoactivity and expression levels of OPN protein were significantly increased in the model group in contrast to the control group(P<0.05).After the intervention,the total score of allergic symptoms,count of lung goblet cells,immunoactivity and expression levels of OPN protein were considerably down-regulated in the catgut implantation group relevant to the model group(P<0.05).H.E.stain showed thickening of partial airway wall,narrowing of lumen,increase of mucus section,widened alveolar septum,infiltration of inflammatory cells,lymphocytes and eosinophil around the bronchus and in the lung interstitium in AR rats,which was milder in the catgut implantation group.The immunoactivity and expression levels of OPN protein were positively related with the lung goblet cells count and lung fiber content(P<0.05,P<0.01).Conclusion Acupoint implantation of catgut can improve pathological changes of lower airway remodeling,which may be related to its effect in down-regulating the expression of OPN protein in the lung tissue.

关 键 词:穴位埋线 过敏性鼻炎 下气道重塑 骨桥蛋白 

分 类 号:R245.9+1[医药卫生—针灸推拿学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象