益气化瘀清热方及其拆方对嘌呤霉素氨基核苷损伤小鼠足细胞表达Podocalyxin及Podocin的影响  

Effect of Yiqi Huayu Qingre Prescription and Its Separations on Expressions of Mice Podocyte Podocalyxin and Podocin by Puromycin Aminonucleoside

作　　者：翟文生[1] 杨濛[1] 张建[1] 张霞[1] 张秋月[1] 张茂华 郭庆寅[1] 宋纯东[1] 李广[1] ZHAI Wensheng;YANG Meng;ZHANG Jian;ZHANG Xia;ZHANG Qiuyue;ZHANG Maohua;GUO Qingyin;SONG Chundong;LI Guang(Pediatrics of The First Afiliated Hospital of Henan Universily of Traditional Chinese Medicine,Zhengzhou 450000,Henan,China;,Jiaozuo Matemal and Child Health Care Hospital,Jiaozuo 454150,Henan,China)

机构地区：[1]河南中医药大学第一附属医院儿科医院肾脏病区,河南郑州450000 [2]焦作市妇幼保健医院,河南焦作454150

出　　处：《中华中医药学刊》2020年第5期25-29,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81273803)。

摘　　要：目的观察益气化瘀清热方及其拆方对嘌呤霉素氨基核苷(Puromycin aminonucleoside,PAN)损伤体外培养的小鼠足细胞表达Podocalyxin及Podocin的影响。探讨不同类中药治疗肾病的作用强度。方法应用PAN(50μg/mL)刺激体外培养的小鼠足细胞,形成PAN足细胞损伤模型,造模成功后的足细胞随机分为益气组、化瘀组、清热组、复方组、对照组、模型组、空白组,采用实时定量逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法(Western Blot)检测各组血清对小鼠足细胞表达Podocalyxin、Podocin的影响。结果益气化瘀清热方及其拆方均能影响造模足细胞Podocalyxin及Podocin的表达,但不同药物作用于造模足细胞的时间不同,其对Podocalyxin及Podocin表达的作用强度不同。结论益气化瘀清热方及其拆方可能通过提高造模足细胞Podocalyxin及Podocin的表达而发挥治疗作用。Objective To observe the effect of Yiqi Huayu Qingre Prescription and its separations on the expressions of mice podocyte Podocalyxin and Podocin and study the drug potency of those traditional Chinese medicine in the treatment of podocyte in vitro stimulated by Puromycin Aminonucleoside(PAN).Methods We stimulated the mice podocyte with PAN(50μg/mL)in vitro to make the model of the damaged podocyte,and the damaged podocyte was divided randomly into seven equal groups:the Yiqi Prescription group,the Huayu Prescription group,the Qingre Prescription group,the Yiqi Huayu Qingre Prescription group,the control group,the model group,the blank group.We observed the effect on the expressions of Podocalyxin and Podocin by using RT-PCR and Western Blot.Results Yiqi Huayu Qingre Prescription and its separations could improve the expressions of Podocalyxin and Podocin,but the work of the different drugs on improving the expressions of modeling podocyte Podocalyxin and Podocin had different effect in the different time.Conclusion Yiqi Huayu Qingre Prescription and its separations may have the treatment on the modeling podocyte by improving the the expressions of Podocalyxin and Podocin.

关 键 词：益气化瘀清热方 足细胞 PODOCALYXIN PODOCIN 

分 类 号：R285.5[医药卫生—中药学]