长链脂肪乳剂预处理对大鼠心肌缺血再灌注损伤的保护作用  

The effect of long chain fat emulsion on the myocardial ischemia reperfusion injury in rats

作　　者：董娇娇[1] 夏芳芳[1] 金周晟[1] 张裕坚[1] 刘乐[1] DONG Jiaojiao;XIA Fangfang;JIN Zhousheng;ZHANG Yujian;LIU Le(Department of Anesthesiology,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China)

机构地区：[1]温州医科大学附属第一医院麻醉科,浙江温州325015

出　　处：《温州医科大学学报》2020年第7期530-534,共5页Journal of Wenzhou Medical University

基　　金：国家自然科学基金资助项目(81670219)。

摘　　要：目的:探讨长链脂肪乳剂预处理对大鼠心肌缺血再灌注(I/R)损伤的保护作用。方法:成年SD大鼠21只,随机分为3组,每组7只;假手术组(Sham组):不进行任何药物处理;模型组(Model组):0.9%氯化钠溶液2 mg·kg^-1·d^-1静脉注射5 d;长链脂肪乳剂组(LE组):Intralipid 2 mg·kg^-1·d^-1静脉注射5 d。末次给药后24 h建立大鼠左前降支结扎I/R模型,其中Sham组只穿线不结扎。持续监测大鼠血流动力学,取心脏进行梗死面积分析,取血检测乳酸脱氢酶(LDH)。结果:与Model组比较,LE组心肌梗死面积占危险区心肌百分比(IR/AAR)和梗死面积占左室面积百分比(IR/LV)显著降低(均P<0.05),LDH释放量显著降低(P<0.05)。与Sham组比较,Model组缺血期及再灌注期平均动脉压(MAP)及心率与收缩压的乘积(RPP)均显著降低(均P<0.05);而LE组和Sham组缺血期及再灌注期MAP及RPP比较差异均无统计学意义(P>0.05)。结论:长链脂肪乳剂预处理能够缩小大鼠心肌I/R损伤后梗死面积、减少LDH释放,同时减缓因I/R损伤所致的血流动力学改变。Objective:To investigate the protective effect of long chain fat emulsion on myocardial ischemia/reperfusion(I/R)injury in rats.Methods:Twenty-one adult SD rats were randomly divided into three groups(n=7):sham operation group(Sham group),no drug treatment;model group(Model group),normal saline 2 mg·kg^-1·d^-1 injection for 5 days;long chain fat emulsion group(LE group),intralipid 2 mg·kg^-1·d^-1 injection for 5 days.Twenty-four hours after the last administration,the model of myocardial ischemia reperfusion(I/R)injury in rats was established by ligating the left anterior descending coronary.Hemodynamics was continuously monitored.The heart was taken for infarction area analysis,and the blood was collected for lactate dehydrogenase(LDH)detection.Results:Compared with the Model group,infarct size divided by area at risk(IR/AAR)and infarct size divided by left ventricle(IR/LV)were significantly reduced in LE group(all P<0.05),and LDH release was markedly lower than that in the Model group(P<0.05).The mean arterial pressure(MAP)and ratepressure product(RPP)of rats in Model group were statistically lower than Sham group in ischemic period and reperfusion period(all P<0.05),while there was no significant difference between LE group and Sham group(P>0.05).Conclusion:Long chain fat emulsion pretreatment can reduce infarct area after myocardial I/R injury in rats,reduce LDH release and alleviate hemodynamic changes caused by I/R injury.

关 键 词：脂肪乳剂 静脉注射 心肌缺血 心肌再灌注损伤 大鼠 

分 类 号：R614[医药卫生—麻醉学]