BALB/c和IFN-γ^-/-小鼠感染虎源弓形虫急性期组织损伤和抗原分布  

Tissue lesions and antigen distribution of BALB/c mice and IFN-γ^-/- mice infected with Toxoplasma gondii isolated from tigers

作　　者：董辉[1] 苏瑞景 杨卫华 杨玉荣[1] DONG Hui;SU Rui-jing;YANG Wei-hua;YANG Yu-rong(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450000,China;Zhengzhou Zhongyuan Region Municipal Agriculture Rural Work Committee,Zhengzhou 450000,China)

机构地区：[1]河南农业大学牧医工程学院,河南郑州450000 [2]郑州市中原区农业农村工作委员会,河南郑州450000

出　　处：《中国兽医学报》2020年第6期1153-1157,共5页Chinese Journal of Veterinary Science

基　　金：河南省高校科技创新人才支持计划资助项目(17HASTIT038);中国博士后科学基金资助项目(2016M600577)。

摘　　要：以BALB/c小鼠和IFN-γ^-/-小鼠为研究对象,应用病理组织学方法,H&E和IHC技术,观察研究TgTigerCHn2(Chinese1,ToxoDB#9)弓形虫感染后急性期死亡小鼠主要器官的组织细胞损伤和虫体分布特点。病理和组织学检查显示,106个TgTigerCHn2速殖子腹腔感染的BALB/c和IFN-γ^-/-小鼠平均生存时间分别约为9.8 DPI(days post infection)和7.7 DPI(P<0.05)。BALB/c小鼠病理表现为脾脏和淋巴结淋巴细胞坏死,弓形虫虫体抗原弥散性分布;间质性肺炎;肝细胞空泡变性及坏死,淋巴细胞弥散性浸润;大脑可见严重的脑膜炎和血管袖套现象。IFN-γ^-/-小鼠主要表现为肝脏有坏死灶,脾脏和淋巴结实质细胞的坏死和减少。IHC显示,BALB/c小鼠大脑的虫体抗原显著多于IFN-γ^-/-小鼠(P<0.01)。结果表明,TgTigerCHn2感染BALB/c小鼠后,虫体主要分布在大脑、肝脏、脾脏、淋巴结和肺脏,而IFN-γ^-/-小鼠各器官表现为炎症反应较轻的坏死性病变。To investigate the characteristics of mice during acute infection with Toxoplasma gondii ToxoDB#9 strain(Chinese1,TgTigerCHn2).The acute mice toxoplasmosis was induced by TgTigerCHn2 tachyzoites intraperitoneally.Serial paraffin sections were made and stained with H&E and IHC,lesions and T.gondii antigen distribution were observed.The average survival time of T.gondii infected BALB/c and IFN-γ^-/-mice was 9.8 days post infection(DPI)and 7.7 DPI,respectively.For BALB/c mice,the lymphoid cells were necrosis and decrease in spleen and mesentery lymph nodes,tachyzoites were diffuse distribution.Interstitial pneumonia,hepatocyte vacuolar degeneration and necrosis,lymphocyte diffuse infiltration,severe meningitis and perivascular infiltration of mononuclear cells in the brain were found.For IFN-γ^-/-mice,diffuse necrosis of liver,necrosis and reduction of parenchymal cells of spleen and mesentery lymph nodes were the main pathological lesions.For brain,IHC showed that BALB/c mice had more significantly T.gondii antigens than IFN-γ^-/-mice(P<0.01).Brain,liver,spleen,lung and mesentery lymph nodes were important target organs after infection with TgTigerCHn2 tachyzoites in BALB/c mice,and necrosis without obvious inflammation was found in IFN-γ^-/-mice.

关 键 词：虎源弓形虫 Chinese1虫株 组织损伤 抗原分布 BALB/c小鼠 IFN-γ^-/-小鼠 

分 类 号：R382.5[医药卫生—医学寄生虫学] R535[医药卫生—基础医学]