玉米单倍体诱导、加倍技术及相关机理探讨  

Analysis of Technique and Mechanism Relate to Induction and Doubling of Haploid in Maize

作　　者：郭书磊[1,2] 魏昕[1] 魏良明[1] 朱卫红[1] 张前进[1] 郭金生[1] 周波[1] 王延昭 韩小花[1] 张新[1] 王振华[1] 陈彦惠[2] 鲁晓民[1] GUO Shu-lei;WEI Xin;WEI Liang-ming;ZHU Wei-hong;ZHANG Qian-jin;GUO Jin-sheng;ZHOU Bo;WANG Yan-zhao;HAN Xiao-hua;ZHANG Xin;WANG Zhen-hua;CHEN Yan-hui;LU Xiao-min(Henan Provincial Key Lab of Maize Biology,Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002;Collaborative Innovation Center of Henan Grain Crops,College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州450002 [2]河南农业大学农学院,郑州450002

出　　处：《玉米科学》2020年第3期52-59,65,共9页Journal of Maize Sciences

基　　金：河南省科技攻关计划项目(No.182102110122);河南省农业科学院自主创新专项基金(No.2019ZC05);中国博士后科学基金(No.2017M612404)。

摘　　要：以生物诱导为基础的DH育种已发展成为玉米育种的核心关键技术,能够显著缩短自交系选育周期,加快育种进程。近年来,单倍体规模化诱导技术已经相当成熟,但批量加倍还是亟待解决的技术难题。文章综述玉米单倍体获得的技术途径、加倍措施、诱导率和加倍率QTL定位及相关基因的研究进展,对单倍体的不同加倍方法以及不同加倍药剂的加倍效果进行分析,通过元分析剖析诱导位点的遗传结构。DH breeding based on biological induction has developed into a key technology of maize breeding,application of DH technology can significantly shorten breeding cycle,improve the breeding efficiency and acceler⁃ate the breeding process.In recent years,the large-scale induction technique of haploid has been quite mature,but batch doubling is still an urgent technical problem.In this paper,new research progress of haploid obtained ap⁃proaches,doubling technical measures,genes or QTLs of induction rate and doubling rate were reviewed;the effec⁃tiveness of different doubling methods and different doubling reagents was also analyzed;furthermore,the genetic ar⁃chitecture of induction locus was dissected by Meta-QTL analysis,which would provide the reference to further re⁃search on haploid breeding technology.

关 键 词：玉米 诱导率 加倍率 Mate-QTL 机理 

分 类 号：S513.035.3[农业科学—作物学]