基于TCGA数据对60岁以上不同分层急性髓系白血病患者相关lncRNA的基因信息学分析  

Genetic Informatics Analysis of lncRNA Related to Patients with Different Stratified Acute Myeloid Leukemia Over 60 Years Based on TCGA Database

作　　者：李悦 徐焕铭[1] 樊华[1] LI Yue;XU Huan-ming;FAN Hua(Department of Hematology,the Fourth Affiliated Hospital of China Medical University,Shenyang 110011,China)

机构地区：[1]中国医科大学附属第四医院血液内科,沈阳110011

出　　处：《现代检验医学杂志》2020年第3期20-25,共6页Journal of Modern Laboratory Medicine

基　　金：辽宁省科学技术项目:核酸切除修复基因xpd及其蛋白在急性白血病中的表达研究[2015020522(F0057)]。

摘　　要：目的通过对肿瘤基因组图谱(the concet genome atlas,TCGA)的相关数据进行分析,以得到长链非编码RNA(long non-coding RNA,lncRNA)及其下游靶分子,对60岁以上急性髓系白血病(acute myeloid leukemia,AML)人群预后影响的相关信息。方法将TCGA数据库中60岁以上AML患者样本,根据美国国立综合癌症网络(NCCN)指南分为高危AML和低中危AML两组。通过R软件中的“edgeR”筛选得出差异表达的lncRNA/miRNA/mRNA。再利用R软件中的“survival”包对在高危AML与低中危AML中存在差异表达的lncRNA进行生存分析,并构建相互竞争的内源性RNA(ceRNA)网络。结果根据TCGA数据库中的lncRNA/miRNA/mRNA表达数据,综合分析得出10个经典lncRNA(AC009154.1,AC011124.1,AC093627.2,AC144450.1,AL035691.1,AL355974.2,AL441943.2,LINC00703,LINC01612和AC103702.2)与60岁以上AML患者预后具有显著的相关性,并得到了与这些lncRNA相关的ceRNA网络。此外,还进行了两者之间存在差异表达的mRNAs基因本体论(gene ontology,GO)和京都基因与基因组百科全书(KEGG)通路分析。结论可以通过干预这些lncRNA的表达,影响高龄AML患者预后,从而延缓或控制高龄AML患者病情的发展。Objective The relevant data of the cancer genome atlas(TCGA)were analyzed to obtain relevant information on the effect of long non-coding RNA(lncRNA)and its downstream target molecules on the prognosis of acute myeloid leakemia(AML)population over 60 years old.Methods AML patients over the age of 60 from the TCGA database were divided into two groups according to the NCCN guidelines:high risk AML and low and medium risk AML.Differentially expressed lncRNA/miRNA/mRNA was screened by“edgeR”in R software.The survival package in R software was used to analyze the survival of lncrnas with differential expression in high-risk AML and low-medium-risk AML,and the ceRNA network was constructed.Results Based on lncRNA/miRNA/mRNA expression data in TCGA database,10 classical lncRNA(AC009154.1,AC011124.1,AC093627.2,AC144450.1,AL035691.1,AL355974.2,AL441943.2,LINC00703,LINC01612 and AC103702.2)were found to have a significant correlation with the prognosis of AML patients over 60 years old after comprehensive analysis,and the ceRNA network related to these lncRNA was obtained.In addition,GO and KEGG pathway analysis of mRNAs with differentially expressed mRNAs were performed.Conclusion By intervening the expression of these lncRNA,the prognosis of elderly AML patients can be affected,thereby delaying or controlling the disease development of elderly AML patients.

关 键 词：急性髓系白血病 长链非编码RNA 肿瘤基因组图谱 竞争内源性RNA 

分 类 号：R557[医药卫生—血液循环系统疾病] Q781[医药卫生—内科学]