黔产犁头草中总黄酮的含量测定  

Content determination of total flavonoidsfrom Viola betonicidolia

作　　者：龚小见[1,2,3] 赵超 周欣[1,2,3] GONG Xiaojian;ZHAO Chao;ZHOU Xin(Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guiyang,Guizhou 550001,China;Guizhou Engineering Laboratory for Quality Control&Evaluation Technology of Medicine,Guizhou Normal University,Guiyang,Guizhou 550001,China;The Research Center for Quality Control of Natural Medicine,Guizhou Normal University,Guiyang,Guizhou 550001,China)

机构地区：[1]贵州省山地环境信息系统与生态环境保护重点实验室,贵州贵阳550001 [2]贵州师范大学贵州省药物质量控制及评价技术工程实验室,贵州贵阳550001 [3]贵州师范大学天然药物质量控制研究中心,贵州贵阳550001

出　　处：《贵州师范大学学报（自然科学版）》2020年第4期40-44,共5页Journal of Guizhou Normal University：Natural Sciences

基　　金：贵州省中药材、民族药材质量标准研究(DBXDYC52071);贵州省高层次创新人才培养项目[黔科合人才(2015)4033号]。

摘　　要：以芦丁为对照品,三氯化铝为显色剂,紫外分光光度(UV)法测定,通过单因素及正交试验对总黄酮的提取方法进行研究。建立黔产犁头草中总黄酮的含量测定方法。芦丁在浓度为0.005~0.04 mg/mL范围内呈良好线性关系,回归方程A=4.290 5C-0.000 4(R^2=0.999 9),平均回收率为97.60%,RSD=0.03%(n=3)。结果表明:贵州不同产地犁头草中总黄酮含量在2.10%~4.04%之间。建立了黔产犁头草中总黄酮含量测定方法,该方法简便快速,回收率高,可为黔产犁头草的质量控制标准提供科学依据。To establish a method for the content determination of total flavones in Viola.betonicidolia from Guizhou,the extraction method of total flavonoids was studied by single factor and orthogonal test with rutin as the reference and aluminum trichloride as the chromogenic agent.Results:There was a good linear relationship between the concentration of rutin in the range of 0.005~0.04 mg/mL.The regression equation was A=4.2905 C-0.0004(R^2=0.9999),and the average recovery was 97.60%,RSD=0.03%(n=3).The content of total flavonoids in Viola betonicidolia from different producing areas in Guizhou ranged from 2.10%to 4.04%.The method was established for the determination of total flavone content in the Viola betonicidolia from Guizhou Province.The method was simple,rapid with high recovery rate,which could provide scientific basis for the quality control standard of the Viola betonicidolia from Guizhou Province.

关 键 词：犁头草 总黄酮 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]