含季铵盐的芳酰腙配体的铜(Ⅱ)配合物的合成和表征:体外DNA键合和核酸酶活性  

Synthesis and Characterization of Copper(Ⅱ)Complexes with Aroylhydrazone Ligands Containing Quaternary Ammonium Salts:in Vitro DNA Binding and Nuclease Activity

作　　者：EdaŞengül Elif Cansu Gökçe Topkaya Tolga Göktürk Ramazan Gup 

机构地区：[1]Department of Chemistry,Faculty of Science,Mugla SıtkıKoçman University,48000,Kotekli-Mugla,Turkey

出　　处：《无机化学学报》2020年第7期1333-1343,共11页Chinese Journal of Inorganic Chemistry

基　　金：the Muğla SıtkıKoçman University Research Projects Coordination Office through Project(Grant No.15/168)。

摘　　要：制备了2个新的含有N,N,N-三甲基丙烷-1-铵基团和N,N,N-三甲基戊烷-1-铵基团的苯乙酮-(4-羟基苯腙)配体:(E)-3-(4-(1-(2-(4-hydroxybenzoyl)hydrazono)ethyl)phenoxy)-N,N,N-trimethylpropan-1-ammonium perchlorate(H2L1)和(E)-3-(4-(1-(2-(4-hy-droxybenzoyl)hydrazono)ethyl)phenoxy)-N,N,N-trimethylpentane-1-ammonium perchlorate(H2L2)。以这2个配体分别合成了2个新的铜配合物:[Cu(HL1)2]和[Cu(HL2)2],研究了这些配体和配合物的DNA结合和切割活性,还研究了DNA切割的机理以及化合物浓度对DNA切割反应的影响。紫外-可见光谱结果表明,所有化合物均优先通过主沟结合模式与DNA结合,在甲基绿(主要的沟结合剂)的存在下切割DNA的活性受到抑制的结果也支持这一结论。电泳研究的结果则表明,化合物在存在或不存在氧化剂(H2O2)的情况下均对质粒DNA表现出显著的切割活性,活性主要取决于化合物的浓度。化合物通过水解途径裂解pBR322 DNA,在存在不同自由基清除剂情况下的DNA裂解实验也支持这一结论。Two new N,N,N-trimethylpropane-1-ammonium and N,N,N-trimethylpentane-1-ammonium conjugated acetophenone-(4-hydroxybenzohydrazone)ligands,(E)-3-(4-(1-(2-(4-hydroxybenzoyl)hydrazono)ethyl)phenoxy)-N,N,N-trimethylpropan-1-ammonium perchlorate(H2L1)and(E)-3-(4-(1-(2-(4-hydroxybenzoyl)hydrazono)ethyl)phenoxy)-N,N,N-trimethylpentane-1-ammonium perchlorate(H2L2),and two new copper complexes with the formulation[Cu(HL1)2]and[Cu(HL2)2]were synthesized and their DNA binding and cleavage activities of the compounds were inves-tigated.The mechanism of DNA cleavage and the effect of the compound concentration on the DNA cleavage reac-tion were also studied.UV-Vis spectroscopy results suggest that all compounds preferably bind to DNA via major groove binding mode.This is also supported that DNA cleavage activity was inhibited in the presence of methyl green,a major groove binder.According to the results of electrophoresis studies,the compounds exhibit significant cleavage activity on the plasmid DNA both in the presence and absence of an oxidative agent(H2O2),which strongly depends on the concentration of the compound.The compounds cleavage pBR322 DNA via hydrolytic pathway,which is also supported by the DNA cleavage experiments in the presence of different radical scavengers.

关 键 词：腙 铜(Ⅱ)配合物 CT-DNA结合 pBR322 DNA切割 

分 类 号：O614.121[理学—无机化学]