肺曲菌病患者外周血中NLRP3-RIP2-NF-κB信号通路的表达及意义  

Expression and Significance of NLRP3-RIP2-NF-κB Signaling Pathway in Peripheral Blood of Patients with Pulmonary Aspergillosis

作　　者：黄应翔 HUANG Ying-xiang(The Department of Respiratory and Critical Care Medicine,Fujian Provincial Geriatric Hospital,Fuzhou350009)

机构地区：[1]福建省老年医院呼吸与危重症医学科,福州350009

出　　处：《实用中西医结合临床》2020年第7期1-3,106,共4页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的:研究NLRP3-RIP2-NF-κB信号通路在肺曲菌病患者外周血中的表达水平,及其介导炎症反应的作用机制。方法:选取2016年5月~2019年10月收治的肺曲菌病患者62例为研究对象,分为侵袭性肺曲霉菌病组(IPA组)24例和非侵袭性肺曲霉菌病组(NIPA组)38例,并选取同期20例非真菌感染者作为对照组。采用RT-PCR法检测三组NLRP3、RIP2、NF-κB mRNA表达水平;Western Blot法检测NLRP3、RIP2、NF-κB蛋白水平;ELISA法检测炎症介质hs-CRP、IL-6水平,对三组上述指标进行比较,并采用Spearman相关性分析分析NLRP3 mRNA与RIP2、NF-κB、IL-6、hs-CRP之间的相关性。结果:(1)IPA组、NIPA组NLRP3、RIP2、NF-κB mRNA表达水平均高于对照组,且IPA组高于NIPA组(P<0.05)。(2)IPA组、NIPA组NLRP3、RIP2、NF-κB蛋白表达水平均高于对照组,且IPA组高于NIPA组(P<0.05)。(3)IPA组、NIPA组IL-6、hs-CRP水平均高于对照组,且IPA组高于NIPA组(P<0.05)。(4)相关性分析发现外周血中NLRP3 mRNA水平与下游基因RIP2、NF-κB mRNA水平呈正相关(相关指数r=0.676,P<0.001;r=0.513,P<0.001);与炎症介质IL-6、hs-CRP及NLRP3水平呈正相关(相关指数r=0.467,P<0.001;r=0.621,P<0.001)。结论:肺曲菌病患者外周血中NLRP3、RIP2、NF-κB mRNA和蛋白表达均升高,NLRP3可能通过RIP2依赖的NF-κB信号通道介导肺曲菌病患者致炎过程。Objective:To study the expression level of NLRP3-RIP2-NF-κB signaling pathway in peripheral blood of patients with pulmonary aspergillosis and its mechanism of mediating inflammatory response.Methods:From May 2016 to October 2019,62 patients with pulmonary aspergillosis were selected as research objects,they were divided into the invasive pulmonary aspergillosis group(IPA group)with 24 cases and the non-invasive pulmonary aspergillosis group(NIPA Group)with 38 cases,and 20 cases of non fungal infection in the same period were selected as the control group.The expression levels of NLRP3,RIP2,NF-κB mRNA were detected by RT-PCR.NLRP3,RIP2 and NF-κB protein levels were detected by Western Blot.The levels of inflammatory mediators hs-CRP,IL-6 were detected by ELISA method.The above indicators of three groups were Compared.The correlation between NLRP3 mRNA,RIP2,NF-κB,IL-6 and hs-CRP was analyzed by Spearman correlation analysis.Results:(1)The mRNA expression levels of NLRP3,RIP2,NF-κB in IPA group and NIPA group were higher than those in control group,and IPA group was higher than those in NIPA group(P<0.05).(2)The protein expression levels of NLRP3,RIP2,NF-κB in the IPA group and the NIPA group were higher than those in the control group,and IPA group was higher than those in NIPA group(P<0.05).(3)The levels of IL-6 and hs-CRP in IPA group and NIPA group were higher than those in the control group,and IPA group was higher than those in NIPA group(P<0.05).(4)correlation analysis showed that NLRP3 mRNA level in peripheral blood was positively correlated with downstream gene RIP2 and NF-κB mRNA levels(correlation index r=0.676,P<0.001;r=0.513,P<0.001);It was positively correlated with levels of inflammatory mediators IL-6,hs-CRP and NLRP3(correlation index r=0.467,P<0.001;r=0.621,P<0.001).Conclusion:The levels of NLRP3,RIP2,NF-κB mRNA and protein expression in peripheral blood of patients with pulmonary aspergillosis were all increased.NLRP3 may mediate the inflammatory process in patients with pulmonary aspergi

关 键 词：肺曲菌病 核苷酸结合寡聚化结构域样受体蛋白3 受体相互作用蛋白2 核因子-ΚB 

分 类 号：R563[医药卫生—呼吸系统]