银杏内酯B改善高脂饮食诱导的小鼠肝脏脂肪变性和炎症  

Ginkgolide B ameliorates high-fat diet-induced hepatic steatosis and inflammation in mice

作　　者：龙思琴 喻秀峰 葛宇黎[1] 刘炉香 Si-Qin Long;Xiu-Feng Yu;Yu-Li Ge;Lu-Xiang Liu(Department of Infectious Diseases,Lishui City People’s Hospital,Lishui 323000,Zhejiang Province,China;Department of Emergency Medicine,Lishui City People’s Hospital,Lishui 323000,Zhejiang Province,China)

机构地区：[1]丽水市人民医院感染科,浙江省丽水市323000 [2]丽水市人民医院急诊科,浙江省丽水市323000

出　　处：《世界华人消化杂志》2020年第13期519-525,共7页World Chinese Journal of Digestology

摘　　要：背景非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)是由于肝脏脂质蓄积诱发的一系列炎症、氧化应激和内质网应激反应导致的的一种慢性肝损伤疾病.银杏内酯B(ginkgolide B,GB)具有抗炎、抗氧化应激和维持脂代谢稳态的作用,提示其可能具有治疗NAFLD的作用.目的探究GB是否对NAFLD具有治疗作用并分析其机制.方法C57BL/6J小鼠分为正常对照(normal control,NC)组、NC+8 mg/kg GB组、高脂饮食(high fat diet,HFD)组、HFD+2 mg/kg GB组、HFD+4 mg/kg GB组和HFD+8 mg/kg GB组.通过HFD诱导法建立NAFLD小鼠模型.收集各组小鼠血液和肝组织并分别进行生化、病理学和Western blot检测.结果病理学结果显示,GB改善HFD导致的肝脏脂肪变性,减少炎症和肝细胞中脂质蓄积.生化结果显示,GB降低HFD喂养的小鼠的血清中谷丙转氨酶、谷草转氨酶、甘油三酯和总胆固醇的水平和肝组织中白介素-6、白介素-1β、肿瘤坏死因子-α的水平.Western blot结果显示,GB抑制HFD诱导的肝组织中真核翻译起始因子2α亚基的磷酸化水平和脂肪酸合酶与活化转录因子表达.结论GB对HFD诱导的NAFLD小鼠具有肝保护作用,且保护作用与其减轻HFD引起的肝损伤、脂肪变性和减弱内质网应激相关.BACKGROUND Nonalcoholic fatty liver disease(NAFLD)is a chronic liver injury caused by a series of inflammation,oxidative stress,and endoplasmic reticulum stress responses induced by lipid accumulation in the liver.Ginkgolide B(GB)has the functions of anti-inflammation,antioxidant stress,and maintenance of lipid metabolism homeostasis,and therefore may have a therapeutic effect on NAFLD.AIM To explore whether GB has a therapeutic effect on NAFLD and analyze its mechanism.METHODS C57BL/6J mice were divided into a normal control group(NC),8 mg/kg GB group,high fat diet(HFD)group,HFD+2 mg/kg GB group,HFD+4 mg/kg GB group,and HFD+8 mg/kg GB group.NAFLD was induced in mice by HFD feeding.Blood and liver tissues were collected for biochemical,pathological,and Western blot analyses.RESULTS Pathological results showed that GB improved hepatic steatosis and reduced inflammatory response and lipid accumulation in liver cells caused by HFD feeding.Biochemical results showed that GB reduced the levels of alanine aminotransferase,aspartate aminotransferase,triglyceride,and total cholesterol in serum as well as the levels of interleukin-6,interleukin-1β,and tumor necrosis factor-αin live tissues in HFD-fed mice.Western blot results showed that GB inhibited the phosphorylation level of eukaryotic translation initiation factor-2α,and the expression of fatty acid synthase and activating transcription factor 4 in liver tissues induced by HFD feeding.CONCLUSION GB has a protective effect on the liver of mice with NAFLD induced by HFD feeding,and the protective effect is related to the reduction of liver damage,steatosis,and endoplasmic reticulum stress.

关 键 词：银杏内酯B 非酒精性脂肪肝病 肝脂肪变性 炎症反应 内质网应激 

分 类 号：R285.5[医药卫生—中药学]