远程缺血中、后处理联合在自发性高血压大鼠心肌缺血再灌注损伤中的作用  

The effect of combined remote conditioning therapy against myocardial ischemia reperfusion injury in spontaneously hypertensive rats

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作  者:鹿欣[1] 陈克彪[1] 张志东[1] 夏涛[1] 毕研玲[2] LU Xin;CHEN Ke-biao;ZHANG Zhi-dong;XIA Tao;BI Yan-ling(Dept.of Cardiac Surgery,Taian City Central Hospital,Taian 271000,China;Dept.of Interventional Radiology,Taian City Central Hospital,Taian 271000,China)

机构地区:[1]泰安市中心医院心脏外科 [2]泰安市中心医院介入放射科,山东泰安271000

出  处:《泰山医学院学报》2020年第7期492-496,共5页Journal of Taishan Medical College

基  金:泰安市科技发展引导计划(2017NS0123)。

摘  要:目的研究远程缺血中处理(RIPER)、远程缺血后处理(RIPOST)及其联合处理对自发性高血压大鼠(SHR)心肌缺血再灌注损伤是否具有保护作用。方法SHR分为服用奥美沙坦药物及未服药两部分,每部分再分5组:伪手术(Sham)组、缺血再灌注(IR)组、远程中处理(RIPER)组、远程后处理(RIPOST)组和联合(RIPER+RIPOST)组。检测各组大鼠左室肥厚指数(LVMI)、肌酸激酶(CK)、缺血和再灌注期间的心率变化、心律失常评分、梗死面积、HIF-1α、miR-21以及miR-210的mRNA表达。结果服用奥美沙坦组大鼠血压降低,左室肥厚及心率失常显著减轻,CK值、梗死面积在RIPER组、RIPOST组和RIPER+RIPOST组中显著减少、HIF-1α、miR-21和miR-210的表达相应的增加。结论RIPER和RIPOST联合处理不能叠加保护缺血心肌、但在抑制凋亡具有叠加保护作用。Objective:To examine whether the combination of remote ischemic perconditioning(RIPER)and remote ischemic postconditioning(RIPOST)can protect myocardium against ischemia reperfusion injury in SHRs.Methods:One hundred SHRs were randomly divided into two groups:Vehicle group and Olmesartan group.Two groups of animals were then assigned to one of the five subgroups:sham group,IR group,RIPER group,RIPOST group and RIPER+RIPOST group.Left ventricular mass index(LVMI),creatine kinase(CK)concentration,heart rate during ischemia and reperfusion,arrhythmia score,infarct size,HIF-1αmRNA expression,miR-21 expression and miR-210 expression were measured.Results:Olmesartan markedly reduced blood pressure and ameliorated left ventricular hypertrophy.CK concentration,infarct size and arrhythmia score during ischemia and reperfusion were significantly improved in the Olmesartan group.The protective effects of RIPER,RIPOST and RIPER+RIPOST were lost in SHRs.HIF-1αexpression miR-21 expression and miR-210 expression expression was significantly increased in the Olmesartan-RIPER group,the Olmesartan-RIPOST group and the Olmesartan-RIPER+RIPOST group in Olmesartan-treated SHRs.Conclusion:The protective effect of the combined remote conditioning therapy is lost in SHR and Olmesartan can restore the protective effect of the combined remote conditioning therapy.The combined remote conditioning therapy gains further advantage in inhibiting apoptosis in Olmesartan-treated SHRs,which may be related to the further upregulations of miR-21 expression and miR-210 expression.

关 键 词:奥美沙坦 自发性高血压大鼠 缺血再灌注损伤 远程缺血中处理 远程缺血后处理 

分 类 号:R602[医药卫生—外科学]

 

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