自身免疫性疾病患者外周血TCR VβCDR3谱型的特点  

Characteristics of TCR V β CDR3 in peripheral blood of patients with autoimmune diseases

作　　者：王春波 王丹[1] 刘海云[1] 王琳[1] WANG Chunbo;WANG Dan;LIU Haiyun;WANG Lin(Department of Rheumatology and rehabilitation,Affiliated Hospital of Liaoning University of traditional Chinese Medicine,Shenyang,Liaoning 110032,China)

机构地区：[1]辽宁中医药大学附属医院风湿康复科,沈阳110032

出　　处：《重庆医学》2020年第S01期10-13,共4页Chongqing medicine

基　　金：辽宁省沈阳市科技局课题(18-013-0-13)。

摘　　要：目的研究自身免疫性疾病(AID)自体外周血T淋巴细胞受体β链(TCRβ)CDR3基因谱型的表达特点,从而总结AID的发病特征与基因谱型的关系及指导免疫治疗。方法选取2007—2009年成都中医药大学附属医院门诊就诊的AID患者41例作为研究对象,应用逆转录-聚合酶链反应扩增AID患者外周血TCRβ可变区(V)基因系列的25个家族,同时将电泳胶于长泳道测序,呈现TCRVβCDR3基因表达谱型图,通过图中电泳条带明确T淋巴细胞质克隆增生特征,分析其TCRβ基因序列,明确AID谱型的特征及AID发病特点并指导AID的治疗。结果CDR3基因表达谱型图呈现10余条呈高斯分布的电泳带,主要由正常TCRβV25个家族的基因序列电泳后依次形成。41例患者CDR3谱型图均呈现异常克隆增生,其中10例系统性红斑狼疮(SLE)患者克隆增生的家族有βV1~4、5.1、6~9、13.1、13.2、14~18、20、21、23~25等,13例类风湿关节炎(RA)患者克隆增生的家族有βV1~4、5.1、5.2、6~9、12、13.1、13.2、14~16、18、20、21、23、24等,4例强直性脊椎炎(AS)患者克隆增生的家族有βV1~4、6、8、9、11、12、14、15、17、20、24、25等,2例白塞氏病患者克隆增生的家族有βV1、3、5.2、11、13.1、13.2、14、15等,6例CTD患者克隆增生的家族除外βV9、18、21、22、24、25等,2例干燥综合征(SS)患者克隆增生的家族有βV2~4、5.2、6、7、13.1、15~17、20等,3例成人STILL′S病患者克隆增生的家族有βV 3、4、5.1、6、8、9、11、13.1、14、15、20等,1例SS患者克隆增生的家族有βV 1、4、13.1、20等。单一条过度浓集之所以在谱型图上呈现是因患者寡克隆T淋巴细胞增殖或单克隆。1例RA患者Vβ25 CDR3区、Vβ25 CDR3区、Vβ4 CDR3区分别与3例SLE、4例SLE、1例SLE患者完全一致。1例AS、1例SLE患者Vβ1 CDR3区完全一致(CASSEGLGAYEQYF),1例SS、1例SLE患者Vβ20 CDR3区完全一致(CAGSVSGRSNNEQFF)。结论AID患者出现T淋巴细胞克隆增生,Objective To study the expression characteristics of CDR3 genotype of T lymphocyte receptorβchain(TCRβ)in peripheral blood of patients with autoimmune diseases(AID),so as to summarize the relationship between the pathogenesis characteristics and the genotype of aid and guide immunotherapy.Methods 41 AID patients from the Affiliated Hospital of Chengdu University of traditional Chinese medicine from 2007 to 2009 were selected as the research objects.25 families of TCRβvariable region(V)gene Series in peripheral blood of AID patients were amplified by reverse transcription polymerase chain reaction.At the same time,electrophoresis gel was sequenced in the long swimlane to present TCR VβCDR3 gene expression pattern.T-lymphocytic clonal hyperplasia was confirmed by electrophoresis strip Characteristics,TCRβgene sequence was analyzed,the characteristics of aid spectrum and the characteristics of AID were clarified,and the treatment of AIDS was guided.Results There are more than 10 bands with Gauss distribution in the CDR3 gene expression pattern,which are mainly formed by the normal TCRβV25 gene sequence after electrophoresis.In 41 patients,abnormal clonal hyperplasia was found in the CDR3 spectrum.In 10 patients with systemic lupus erythematosus(SLE),the families of clonal hyperplasia wereβV1-4,5.1,6-9,13.1,13.2,14-18,20,21,23-25,etc.in 13 patients with rheumatoid arthritis(RA),the families of clonal hyperplasia wereβV1-4,5.1,5.2,6-9,12,131,13.2,14-16,18,20,21,23,24 and so on.4 patients with as hadβV1-4,6,8,9,11,12,14,15,17,20,24,25 and so on.2 patients with Behcet's disease hadβV1,3,5.2,11,13.1,13.2,14,15 and so on.6 patients with CTD hadβV9,18,21,22,24 and so on.2 patients with Sjogren's syndrome(SS)hadβV2-4,5.2,6,7,13.1,15,16,17,20,etc.3 patients with adult still's disease hadβV3,4,5.1,6,8,9,11,13.1,14,15,20,etc.1 patient with SS hadβV1,4,13.1,20,etc.The reason for the over concentration of a single stripe on the spectrum is the proliferation or monoclonal of the patient's oligoclonal T lymphocytes.V

关 键 词：自身免疫性疾病 T淋巴细胞受体 T淋巴细胞受体β链可变区 CDR3基因 

分 类 号：R59[医药卫生—内科学]