黑果枸杞多糖对大鼠肝损伤的保护研究  

Protective Effect of Lycium barbarum polysaccharide on Hepatic Injury by Acrylamide in Rats

作　　者：王莉[1] 叶维佳 吴红静[1] 刘健 庞旭涛 林芝雨 WANG Li;YE Wei-jia;WU Hong-jing;LIU Jian;PANG Xu-tao;LIN Zhi-yu(College of Science and Technology,Nanchang University,Nanchang 330029,China)

机构地区：[1]南昌大学科学技术学院,江西南昌330029

出　　处：《食品工业科技》2020年第14期287-290,296,共5页Science and Technology of Food Industry

基　　金：江西省教育厅科学技术研究项目(171456)。

摘　　要：目的:探讨黑枸杞多糖(LBP)对丙烯酰胺(AA)致大鼠肝损伤的保护作用。方法:取健康成年大鼠70只,随机分为7组:染毒模型组(AA,灌胃AA水溶液20 mg/kg bw)、阳性对照组(NAC+AA,灌胃AA水溶液20 mg/kg bw和N-乙酰半胱氨酸水溶液200 mg/kg bw)、毒性抑制组(LBP+AA,分黑枸杞多糖低、中、高剂量组,灌胃AA水溶液20 mg/kg bw后分别灌胃黑枸杞多糖50、100、150 mg/kg bw)、高剂量多糖组(LBPH,灌胃黑枸杞多糖200 mg/kg bw)、正常对照组(CT,给予同等体积生理盐水)。连续给药饲喂4周,实验期间每周对大鼠进行称重,结束给药后进行各类相关指标测定及观察肝脏组织结构。结果:与CT组相比较,AA染毒组大鼠体重减少显著,大鼠肝组织细胞内SOD、CAT、GSH-Px的含量均呈显著性降低,MDA、AST、ALT的含量显著增高,差异均具有统计学意义(P<0.05)。AA染毒组肝组织出现严重病变,肝小叶排列紊乱,肝细胞之间边缘界限模糊;与AA染毒组相比较,摄入不同浓度LBP实验组与NAC阳性对照组大鼠体重都得到明显改善,细胞中SOD、CAT、GSH-Px的活力均有显著性提高,肝组织中MDA、AST、ALT的含量均显著性降低,差异均具有统计学意义(P<0.05)。NAC+AA组和LBP+AA各组肝组织病变情况得到很好的改善,肝细胞没有明显的病例症状,肝细胞排列正常,细胞边缘界限清楚,细胞结构完整。结论:黑枸杞多糖能够改善AA染毒大鼠的肝损伤,提高肝脏抗氧化水平。Objective:To investigate the protective effect of LBP on liver injury induced by acrylamide(AA)in rats.Methods:70 healthy adult rats were randomly divided into 7 groups:AA infected group(AA,gavaged AA aqueous solution 20 mg/kg bw),positive control group(NAC+AA,gavaged AA aqueous solution 20 mg/kg bw and N-acetyl cysteine aqueous solution 200 mg/kg bw),toxic suppression group(LBP+AA,divided into low,medium and high dose groups,gavaged AA aqueous solution 20 mg/kg bw and LBP 50 mg/kg bw,100 mg/kg bw,150 mg/kg bw),polysaccharide high dose group(LBPH,gavaged LBP 200 mg/kg bw)and normal group(CT,gave the same volume of saline).The drugs was given continuously for 4 weeks,the rats were weighed weekly,after administration,various related indexes were measured and liver tissue structure was observed.Results:The weight loss of rats in the AA-infected group was significant,the SOD,CAT and GSH-Px contents in the liver tissue of the rats were significantly decreased,and the contents of MDA,AST and ALT were significantly increased compared with the CT group(P<0.05).Severe pathological changes were observed in liver tissues in the AA-infected group,with disordered arrangement of hepatic lobules and blurred boundary between hepatocytes.The weight of rats in the LBP groups and the NAC group were significantly improved,the activity of SOD,CAT,GSH-Px in cells were significantly increased,and the contents of MDA,AST and ALT in liver tissues were significantly decreased compared with the AA infected group(P<0.05).The pathological changes of liver tissues were improved,the liver cells were arranged normally,the cell boundaries were clear and the cell structure was complete in NAC+AA group and LBP+AA groups.Conclusion:Lycium barbarum polysaccharide can improve the liver injury of AA infected rats and improve the level of anti-oxidation in the liver.

关 键 词：黑枸杞多糖 丙烯酰胺 肝损伤 保护作用 

分 类 号：TS201.4[轻工技术与工程—食品科学]