复方丹参片及其拆方对心肌缺血大鼠模型的影响  

Effect of Compound Danshen Tablet and its disassembled formula on myocardial ischemia rat model

作　　者：李晓霞 李新 林娟[3] 王德勤[3] 周福军[2] 匡艳辉[3] 黄启和 梁小娜 徐旭[2,6,7] 郭海彪 LI Xiaoxia;LI Xin;LIN Juan;WANG Deqin;ZHOU Fujun;KUANG Yanhui;HUANG Qihe;LIANG Xiaona;XU Xu;GUO Haibiao(North China University of Science and Technology,Tangshan 063200,Hebei,China;Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China;Guangzhou Baiyunshan Hutchison Whampoa Chinese Medicine Co.,Ltd,Guangzhou 510515,China;Tianjin Medical University,Tianjin 300070,China;Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin 300193,China;Tianjin Key Laboratory of Quality Markers for Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]华北理工大学,河北唐山063200 [2]天津药物研究院,天津300193 [3]广州白云山和记黄埔中药有限公司,广东广州510515 [4]天津医科大学,天津300070 [5]天津中医药大学,天津300193 [6]释药技术与药代动力学国家重点实验室,天津300193 [7]天津市中药质量标志物重点实验室,天津300193

出　　处：《药物评价研究》2020年第6期1026-1032,共7页Drug Evaluation Research

基　　金：广东省重点领域研发计划项目(2020B1111110002);国家中医药管理局中药标准化项目(ZYBZH-C-GD-05);天津市科技计划项目(15ZXXYSY00070)。

摘　　要：目的观察复方丹参片及其拆方对冠心病心肌缺血大鼠模型的影响。方法SD大鼠随机分为假手术组,模型组,复方丹参片高、低剂量(0.6、0.3 g/kg)组,单硝酸异山梨酯片(阳性药,6.9 mg/kg)组,丹参提取物(0.3 g/kg)组,三七粉(0.3 g/kg)组,ig给药,每日1次,预防给药7 d,末次给药1 h后结扎冠脉左前降支制备心肌缺血大鼠模型。检测大鼠结扎前及结扎后Ⅱ导心电图,观察ST段变化值;最后一次心电图检测后腹主动脉取血,ELISA法检测血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、天门冬氨酸转氨酶(AST)、一氧化氮(NO)、一氧化氮合成酶(eNOS)、内皮素(ET)、血栓素(TXB2)、前列环素(6-keto-F1α)水平;取心脏,TTC染色检测心肌缺血率。结果与对照组比较,模型组心电图ST段显著抬高,心肌缺血明显,血清心肌酶AST、LDH、CK-MB水平均显著升高,TXB2水平显著升高,6-keto-F1α水平显著降低,NO、eNOS水平显著降低,ET水平显著升高(P<0.01、0.001)。与模型组比较,复方丹参片高、低剂量组心电图ST段抬高显著改善,心肌缺血率显著降低,血清各生化指标均显著改善(P<0.05、0.01);丹参提取物组心电图ST段抬高,心肌缺血率,血清CK-MB、LDH、eNOS、ET、TXB2、6-keto-F1α水平均显著改善(P<0.05),AST和NO水平差异无统计学意义;三七粉组心电图ST段、血清CK-MB、ET、TXB2、6-keto-F1α显著改善(P<0.05),心肌缺血率和AST、LDH、NO、eNOS水平差异无统计学意义。结论在改善冠心病大鼠心肌缺血方面,复方丹参片效果较单味药好,丹参提取物效果较三七粉效果较好。Objective To observe the effect of Compound Danshen Tablet and its disassembled formula on the rat model of myocardial ischemia of coronary heart disease.Methods SD rats were randomly divided into sham operation group,model group,high and low dose groups(0.6 g/kg,0.3 g/kg)of Compound Danshen Tablet,positive drug group(isosorbide mononitrate 6.9 mg/kg),Danshen Extract group(0.3 g/kg),Sanqi powder group(0.3 g/kg).Rats were continuous administrated by gavage once a day for 7 days.One hour after the last administration,the left anterior descending of coronary artery(LAD)of rat was ligated to prepare the model of myocardial ischemia.Before and after ligation,the ECG of rat was respectively measured at 5,15,30,45,60,90,120,180 min and 24 h,and the change of ST segment was determined.After the last ECG,the blood was taken from abdominal aorta,then detecting the level of CK-MB,LDH,AST,NO and eNOS,ET,TXB2,and 6-keto-F1αby ELISA assay.And the heart was taken to detect the infarct area.Results Compared with control group,the ST segment of ECG in the model group was significantly elevated,myocardial ischemia was significant,serum myocardial enzyme AST,LDH,CK-MB levels were significantly increased,TXB2 level was significantly increased,6-keto-f1αlevel was significantly reduced,NO,eNOS level was significantly reduced,ET level was significantly increased(P<0.01,0.001).Compared with the model group,the ECG on the high and low dose group of Compound Danshen Tablet was significantly improved,as well as the index of vasomotor factor.Furthermore,Compound Danshen Tablet could decrease the serum myocardial enzyme and the antithrombotic index,the difference was statistically significant(P<0.01).ST segment of ECG,CK-MB,LDH,eNOS,ET,TXB2,6-keto-F1αin Danshen Extract group were significantly improved(P<0.05),while AST and NO had no significantly difference.The other formula,Sanqi could significantly improve the ST segment of ECG,CK-MB,ET,TXB2 and 6-keto-F1α(P<0.05),but AST,LDH,NO and eNOS were not significantly changed.Conclusion Accor

关 键 词：复方丹参片 丹参提取物 三七粉 冠心病 心肌缺血 心电图 心肌酶 血管舒缩因子 

分 类 号：R285.5[医药卫生—中药学]