不同寄主来源的Monilinia fructicola 对多菌灵的敏感性及其遗传结构特征  

Sensitivity of Monilinia fructicola from different hosts to carbendazim and features of population structure

作　　者：陈凤平[1] 张玥[1] 周挺[2] 李雄 刘西莉[3,4] CHEN Fengping;ZHAGN Yue;ZHOU Ting;LI XIONG;LIU Xili(Department of Plant Pathology,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Tobacco Science,Fujian Provincial Tobacco Company,Fuzhou 350003,China;Department of Plant Pathology,China Agricultural University,Beijing 100193,China;Department of Plant Pathology,Northwest A&F University,Yangling 712100,Shaanxi Province,China)

机构地区：[1]福建农林大学植物病理学系,福州350002 [2]中国烟草总公司福建省公司,福州350003 [3]中国农业大学植物病理学系,北京100193 [4]西北农林科技大学植物病理学系,陕西杨凌712100

出　　处：《农药学学报》2020年第3期423-431,共9页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金(31972294);国家重点研发项目(2016YFD0201305).

摘　　要：美澳型核果链核盘菌Monilinia fructicola(G.Winter)Honey是引起多种果树褐腐病的重要病原菌,多菌灵是防治该病害的重要杀菌剂。为明确不同寄主来源的菌株对多菌灵的敏感性及遗传结构差异,研究测定了来源于樱桃、李子和毛桃的17株菌株对多菌灵的敏感性,同时基于Tub2核苷酸序列分析了来源于樱桃和毛桃的32株M.fructicola群体的遗传多样性和群体分化特征。结果表明:来自樱桃、李子和毛桃的17株菌株中,多菌灵的EC50<1μg/mL和>1μg/mL的菌株比例分别为58.8%和41.2%,其中EC50>50μg/mL的菌株比例为17.6%。来自毛桃的菌株群体多样性比来自樱桃的高,其核苷酸多样性分别为3.25×10^−3和0.94×10^−3,单倍型多样性分别为0.883和0.242;两群体间存在显著的遗传分化,分化程度较高,其FST值达到0.148。单倍型网络分析结果显示:来自樱桃和毛桃的群体分别含有3种和9种单倍型,其中共有单倍型2种;不同单倍型在进化过程中主要发生了两个途径的进化,其中一个途径只出现了来自毛桃群体的1种单倍型,其他单倍型经过不同的突变步骤形成另一个途径。寄主特异性检验结果发现,来自樱桃的菌株具有一定的寄主特异性,而来自毛桃的菌株与寄主的关联性较差。综合研究表明,M.fructicola对多菌灵的抗性频率较高,且来自不同寄主的群体遗传结构差异性较大。Monilinia fructicola is an important pathogen,causing brown rot in many fruit trees.Carbendazim is an effective fungicide for the control of this disease.In order to clarify the sensitivity of M.fructicola to carbendazim,and to understand the the genetic structure of this pathogen originated from different hosts,the sensitivities of 17 isolates from cherry,plum and peach were investigated in this study.The genetic diversity and genetic differentiation of 32 M.fructicola isolates from cherry and peach were also analyzed accroding to Tub2 nucleotide sequences.The results showed that the proportion of isolates with EC50<1μg/mL and>1μg/mL was 58.8%and 41.2%,respectively,among which the proportion of isolates with EC50>50μg/mL was 17.6%.The population diversity of M.fructicola from peach was higher than that from cherry,with the nucleotide diversity of 3.25×10^−3 and 9.04×10^−3,respectively,and the haplotype diversity of 0.883 and 0.242,respectively.Significant genetic differentiation between the two populations was detected,and the degree of differentiation was medium with the FST value of 0.148.The haplotype network analysis showed that the populations from cherry and peach contained 3 and 9 haplotypes,respectively,within which 2 haplotypes were common.Different haplotypes evolved mainly in two ways.One pathway only showed one haplotype from peach population,and the other haplotypes formed the second pathway through different mutation steps.The host-specific test showed that the isolates from cherry had a certain host specificity,while the isolates from peach were less associated with the host.In conclusion,the resistant frequency of M.fructicola to carbendazim was high and the genetic structure of populations from different hosts was quite diverse.

关 键 词：美澳型核果链核盘菌 褐腐病 多菌灵 遗传多样性 遗传结构 

分 类 号：S482.2[农业科学—农药学]