针刺调节炎性细胞因子改善大鼠脑梗死急性期脑血流的USPIO-DSC MRI研究  

USPIO-DSC MRI study on acupuncture improving cerebral blood flow of pMCAO rats by regulating levels of inflammatory cytokines

作　　者：杨烁慧[1] 吴瑜 詹松华[1] 杨辰瑶[1] 孔营楠 张成 陆方[1] YANG Shuohui;WU Yu;ZHAN Songhua;YANG Chenyao;KONG Yingnan;ZHANG Cheng;LU Fang(Department of Radiology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院放射科,上海201203 [2]上海中医药大学,上海201203

出　　处：《磁共振成像》2020年第7期557-562,共6页Chinese Journal of Magnetic Resonance Imaging

基　　金：国家自然科学基金青年项目(编号:81403204);国家自然科学基金面上项目(编号:81573782)。

摘　　要：目的应用超微超顺磁性氧化铁(ultrasmall superparamagnetic particles of iron oxides,USPIO)动态磁敏感对比增强(susceptibility-weighted contrast-enhanced,DSC)MRI评价针刺通过调节血清炎性细胞因子水平以改善永久性大脑中动脉栓塞(permanent middle cerebral artery occlusion,pMCAO)模型大鼠急性期梗死区域脑血流量和脑血容量的有效性。材料与方法首先建立24只SD大鼠pMCAO和假手术组模型:假手术组6只(A组)、无电针梗死组10只(B组)、电针梗死组8只(C组)。每组再分为24 h和48 h二个亚组。造模成功后C组大鼠选取百会穴、右侧足三里穴和水沟穴进行电针治疗。每只大鼠在相应时间点在1.5 T MRI上行USPIO-DSC MRI扫描。测量模型鼠梗死区域的相对脑血容量(relative cerebral blood volume,rCBV)和相对脑血流量(relative cerebral blood flow,rCBF),测量对侧镜像区域相应参数值,并计算比值(rrCBV和rrCBF)。观察24 h和48 h A组、B组和C组间的rrCBV、rrCBF值有无显著差异,以及B组加C组的这二个参数值分别与白介素(interleukin,IL)-1β、IL-6、IL-10和肿瘤坏死因子(tumor necrosis factor-α,TNF-α)是否相关。结果A组rrCBV和rrCBF在24 h和48 h均显著高于B组和C组(P<0.05)。C组rrCBV和rrCBF在24 h和48 h均显著高于B组(P<0.05)。24 h B组加C组rrCBV、rrCBF分别与IL-1β、IL-6显著负相关(P<0.05),与IL-10显著正相关(P<0.05);rrCBV与TNF-α显著正相关(P<0.05)。48 h B组加C组rrCBV、rrCBF分别与IL-1β显著负相关(P<0.05),与IL-10显著正相关(P<0.05)。结论针刺在下调炎性因子IL-1β、IL-6以减轻炎性反应的同时,又通过上调IL-10和TNF-α来增加pMCAO大鼠急性期梗死区域的rrCBV和rrCBF,以维持梗死区域的血供。Objective:To evaluate the effectiveness of acupuncture on improving CBF and CBV within infarcted areas during acute-stage by regulating serum levels of inflammatory cytokines in permanent middle cerebral artery occlusion(pMCAO)model rats with ultrasmall superparamagnetic particles of iron oxides(USPIO)dynamic susceptibility contrast(DSC)MRI.Materials and Methods:Twenty-four SD rats models were established,including sham-operation group(group A,n=6),pMCAO group without acupuncture(group B,n=10),pMCAO group with electroacupuncture(group C,n=8).Each group was further divided into 2 subgroups according to 24 h and 48 h after modeling.Rats in the group C were treated with electroacupuncture on Baihui,Shuigou and right Zusanli acupoints.Every rat in each group was scanned with USPIO-DSC on a 1.5 T MR scanner.The values of the relative cerebral blood volume(rCBV)and the relative cerebral blood flow(rCBF)on regions of infarct and contralateral in mirror areas were measured.The ratios(infarct/contralateral)of rrCBV and rrCBF were calculated.These ratios on 24 h and 48 h were compared to evaluate whether there were any statistical differences among Group A,B and C.Spearman correlation coefficients were calculated between the ratios rrCBV and rrCBF of Group B plus C and values of IL-1β,IL-6,IL-10 and TNF-α,respectively.Results:The values of rrCBV and rrCBF were significantly higher in group A than those in group B and C on 24 h and 48 h(P<0.05).The values of rrCBV and rrCBF were significantly higher in group C than those in group B on 24 h and 48 h(P<0.05).On 24 h,the values of rrCBV and rrCBF of group B plus C were significantly negatively correlated with the values of IL-1βand IL-6,and positively correlated with the values of IL-10(P<0.05).The values of rrCBV of group B plus C were significantly positively correlated with the values of TNF-αon 24 h(P<0.05).On 48 h,the values of rrCBV and rrCBF of group B plus C were significantly negatively correlated with the values of IL-1β,and positively correlated with the values

关 键 词：超微超顺磁性氧化铁 磁共振成像 炎性细胞因子 针刺 脑梗死 

分 类 号：R445.2[医药卫生—影像医学与核医学] R743.33[医药卫生—诊断学]