钙离子载体对卵胞质内单精子显微注射受精失败者卵母细胞的激活作用  

Activation effects of calcium ionophore on the oocyte after failure of intracytoplasmic sperm injection fertilization

作　　者：孙玲 杨险峰[1] 娄华[1] 刘汝升 刘杨青 高松占 Sun Ling;Yang Xianfeng;Lou Hua;Liu Rusheng;Liu Yangqing;Gao Songzhan(Reproductive Medicine Center,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第三附属医院生殖中心,450000

出　　处：《中国实用医刊》2020年第1期11-14,共4页Chinese Journal of Practical Medicine

摘　　要：目的 观察使用钙离子载体A23187行人工辅助激活技术对受精低下或受精失败的患者再次行单精子卵细胞质内注射(ICSI)助孕时,患者成熟卵母细胞的受精率、卵裂率及胚胎结局.方法 选择2015年1月至2015年12月在郑州大学第三附属医院生殖医学中心行助孕治疗的患者11例,均因上1个周期行ICSI受精失败或者受精低下(受精率<30%)而再次行ICSI助孕治疗,对其临床资料进行回顾性分析.将使用钙离子载体A23187进行激活处理的11例患者的122枚成熟卵母细胞作为激活组,将11例入选患者于上1个周期按照常规ICSI操作处理的108枚卵母细胞作为对照组,观察使用钙离子载体对卵母细胞激活后的受精率、卵裂率及出生子代的一般情况.结果 激活组受精率及卵裂率分别为36.9%(45/122)、88.9%(40/45),对照组受精率和卵裂率分别为12.5%(21/108)、61.9%(13/21),差异有统计学意义(P<0.05);激活组中可利用胚胎数[(3.42±2.69)枚]及优质胚胎数量[(1.67±1.24)个]较对照组[(1.51±0.53)枚、(0.76±0.54)个]均显著提高(P<0.05).11例患者中,卵母细胞激活处理后5例患者获得临床妊娠.结论 使用钙离子载体A23187对成熟卵母细胞进行人工辅助激活,可以改善ICSI受精失败或受精低下患者的受精率、卵裂率及妊娠率.Objective To observe the fertility rate,cleavage rate and embryo outcome in the second time of intracytoplasmic sperm injection(ICSI)with artificial assisted activation by calcium ionophore A23187 in women who once had low or failed fertilization.Methods Eleven women with assisted pregnancy in the Reproductive Medicine Center of the Third Affiliated Hospital of Zhengzhou University from January 2015 to December 2015 were selected.All of them were treated by ICSI again due to the failure of fertilization or low fertilization rate(fertilization rate<30%)of ICSI in the last cycle.The clinical data of all patients were analyzed retrospectively.A total of 122 mature oocytes from 11 patients activated by calcium ion carrier A23187 were used as the activation group,and 108 oocytes from the 11 patients in the previous conventional ICSI operation were used as the control group.The fertilization rate,cleavage rate and general situation of the infants born after the activation of oocytes with calcium ion carrier were observed.Results The fertility rate and cleavage rate of the activation group were 36.9%(45/122)and 88.9%(40/45),respectively;and they were 12.5%(21/108)and 61.9%(13/21),respectively,of the control group,and the difference was statistically significant between the two groups(P<0.05).The number of available embryos and the number of high quality embryos in the activation group were 3.42±2.69 and 1.67±1.24,significantly higher than the 1.51±0.53 and 0.76±0.54 in the control group,and the difference was statistically significant(P<0.05).In the 11 patients,5 cases got clinical pregnancy after oocyte activation.Conclusions Artificial assisted activation for mature oocytes by the calcium ionophore A23187 can improve the fertility rate,cleavage rate and pregnancy rate of patients once with failed ICSI fertilization or fertilization rate.

关 键 词：卵母细胞 钙离子载体 单精子卵细胞质内注射 受精失败 

分 类 号：R714[医药卫生—妇产科学]