独子藤根皮醋酸乙酯部位的化学成分研究  

Chemical constituents of ethyl acetate extracts from root bark of Celastrus monospermus

作　　者：陈铭祥 关睿 廖泽纯 宋晶晶 胡爱娣 郑明彬 CHEN Ming-xiang;GUAN Rui;LIAO Ze-chun;SONG Jing-jing;HU Ai-di;ZHENG Ming-bin(School of Pharmacy,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学药学院,广东东莞523808

出　　处：《中草药》2020年第11期2895-2899,共5页Chinese Traditional and Herbal Drugs

基　　金：广东省中医药局面上科研项目(20171154);湛江市科技攻关计划项目(2017B01062);广东医科大学科研基金面上项目(M2016005);广东医科大学大学生创新训练项目(GDMU2017068,GDMU2017115);广东省大学生创新训练项目(201810571068,201810571115)。

摘　　要：目的研究独子藤Celastrusmonospermus根皮醋酸乙酯部位的化学成分。方法采用硅胶、反相硅胶和凝胶等柱色谱方法进行分离纯化,通过波谱学数据鉴定化合物的结构。结果从独子藤根皮醋酸乙酯部位中分离得到12个化合物,分别鉴定为美登木酸(1)、对羟基苯甲酸(2)、4-羟基-3-甲氧基苯甲酸(3)、大子五层龙酸(4)、3-氧代-2β-羟基木栓烷-29-酸(5)、雷公藤红素(6)、3α,22β-二羟基齐墩果烷-12-烯-29-酸(7)、直楔草酸(8)、21-氧代-2α,3α,22β-三羟基-29-降-木栓烷-24,2β-内酯(9)、2,3-二氧代-6α,10-二羟基-24-降-木栓烷-4,7-二烯-29-酸(10)、2α,3β,19α,23-四羟基-齐墩果烷-12-烯-28-酸(11)、2α,3β-二羟基-齐墩果烷-12-烯-23,28,30-三酸(12)。结论化合物1、3、7、10~12为首次从本植物中分离得到,3、11、12为首次从本属植物中分离得到。Objective To study the chemical constituents of ethyl acetate extracts from the root bark of Celastrus monospermus. Methods The compounds were separated and purified by repeated column chromatographic methods including silica gel, ODS and Sephadex LH-20, and the chemical structures of compounds were determined by spectral data analyses. Results Twelve compounds were obtained and identified as polpunonic acid(1), p-hydroxybenzoic acid(2), 4-hydroxy-3-methoxybenzoic acid(3), salaspermic acid(4), 3-oxo-2β-hydroxyfriedelan-29-oic acid(5), celastrol(6), 3α,22β-dihydroxy-olean-12-en-29-oic acid(7), orthosphenic acid(8), 21-oxo-2α,3α,22β-trihydroxy-29-nor-friedelan-24,2a-lactone(9), 2,3-dioxo-6α,10-dihydroxy-24-nor-friedelan-4,7-dien-29-oic acid(10), 2α,3β,19α,23-tetrahydroxyolean-12-en-28-oic acid(11) and 2α,3β-dihydroxyolean-12-en-23,28,30-trioic acid(12). Conclusion Compounds 1, 3, 7, 10-12 are obtained from this plant for the first time, and compounds 3, 11, 12 of them were separated from the genus Celastrus for the first time.

关 键 词：独子藤 醋酸乙酯部位 美登木酸 4-羟基-3-甲氧基苯甲酸 2α 3β 19α 23-四羟基-齐墩果烷-12-烯-28-酸 

分 类 号：R284.1[医药卫生—中药学]