基于de novo高通量测序的叶尔羌高原鳅微卫星位点筛选与多态性分析  

Microsatellite Screening and Characterization Analysis of Triplophysa Yarkandensis based on the Whole Genome de novo Sequencing

作　　者：王锦秀 宋勇[1,2] 王新月 陈生熬[1,2] 任道全[1,2] Wang Jinxiu;Song Yong;Wang Xinyue;Chen Shengao;Ren Daoquan(College of Animal Science,Tarim University,Alar,Xinjiang 843300;Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production&Construction Corps.Alar,Xinjiang 843300)

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2020年第2期25-32,共8页Journal of Tarim University

基　　金：国家自然科学基金项目“生境破碎化对叶尔羌高原鳅种群遗传结构的影响”(31460691);塔里木大学研究生科研创新项目“叶尔羌高原鳅微卫星标记的筛选”(TDGRI201821)。

摘　　要：为保护该鱼类资源而开发塔里木河流域叶尔羌高原鳅(Triplophysa yarkandensis)群体的微卫星标记,合成二、三碱基重复类型的100对引物,筛出33对多态性引物,进一步分析其多态性。结果显示:102尾叶尔羌高原鳅,其平均等位基因和有效等位基因分别为43.848和12.163,平均观测杂合度(Ho)为0.239,平均期望杂合度(He)平均值为0.902,多态性信息含量PIC数值为0.890。碱基重复类型中二碱基重复次数在6~61次,三碱基在5~25次,四碱基在7~27次、五碱基在6~12次,六碱基在5~17次。研究发现,碱基数越多,重复次数越少,且随着碱基数量的增加,相应微卫星标记的数量也逐渐减少。同时表明二碱基重复的微卫星标记明显比三碱基重复类型的微卫星标记多态性指数高。The study sequenced and synthesized 100 pairs of microsatellite primers with double and triple base repeat through high throughput sequencing in order to develop microsatellite markers of the populations of Triplophysa yarkandensis in the Tarim river basin.Finally,33 pairs of primers with high polymorphism were selected.Polymorphism analysis was performed on 102 Triplophysa yarkandensis using 33 pairs of primers obtained.The results showed that,the average allele and the effective allele were 43.848 and 12.163.The mean observed heterozygosity(Ho)and expected heterozygosity(He)were 0.239 and 0.902,respectively.The polymorphism information content(PIC)was 0.890.In the sequence of microsatellite,the more the base number,the less the repetition times,and with the increase of the base number,the corresponding number of microsatellite markers gradually decreased.The polymorphism index of SSR markers of dibasic repeat was significantly higher than that of trinucleotide repeat.

关 键 词：叶尔羌高原鳅 微卫星 二三碱基重复 多态性 

分 类 号：S932.4[农业科学—渔业资源]