基于网络药理学探讨涤痰汤治疗脑梗死作用机制  

Exploring Mechanism of Ditan Decoction(涤痰汤)in Treating Cerebral Infarction Based on Network Pharmacology

作　　者：马岱朝 陈会生 刘辉[1] MA Daichao;CHEN Huisheng;LIU Hui(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;North Theater General Hospital,Shenyang 110016,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]北部战区总医院,辽宁沈阳110016

出　　处：《山东中医药大学学报》2020年第4期350-355,共6页Journal of Shandong University of Traditional Chinese Medicine

基　　金：科技部慢病专项基金项目(编号:2017YFC1308200);辽宁省科技攻关项目(编号:2018225023)。

摘　　要：目的:探讨涤痰汤治疗脑梗死潜在的分子机制。方法:采用网络药理学中药物成分的靶点预测、疾病相关靶点的数据挖掘、蛋白互作网络的生物功能模块分析、信号通路的富集、可视化网络构建等方法,分析涤痰汤治疗脑梗死的蛋白靶点、生物过程、信号通路。结果:得到124个靶点,这些靶点构成的蛋白互作网络的生物功能模块参与凝血途径的激活、血凝块形成、纤溶系统激活、炎症反应、活性氧簇的生物合成等生物过程。涤痰汤可能作用于白细胞介素-17(IL-17)信号通路、缺氧诱导因子-1(HIF-1)信号通路、核转录因子-Kappa B(NF-κB)信号通路、Toll样受体(TLRs)信号通路、肿瘤坏死因子(TNF)信号通路、血小板激活、补体与凝血瀑布通路并通过干预脑梗死后的脑水肿形成、炎症反应、血管再生、血栓形成和补体系统激活等方式发挥治疗作用。结论:揭示了涤痰汤治疗脑梗死潜在的靶点、生物功能模块、京都基因与基因组百科全书(KEGG)信号通路,为进一步研究提供了理论依据和方向。Objective:To explore the potential molecular mechanism of Ditan Decoction(涤痰汤)for treating cerebral infarction.Methods:Network pharmacological methods were used to predict components’targets,mine related targets within databases,analyze biological function modules of protein interaction network,enrich targets’pathway and construct visual networks.The molecular targets,biological process and signaling pathways of Ditan Decoction to cure cerebral infarction were analyzed.Results:One hundred and twenty-four targets were found.Biological function module of the protein interaction network formed by these targets,involves in biological processes like activation of coagulation pathway,formation of the blood clot,activation of fibrinolytic system,inflammatory response,synthesis of reactive oxygen species.By impacting on the signaling pathways of interleukin-17(IL-17),hypoxia-inducible factor-1(HIF-1),nuclear transcription factor kappa B(NF-κB),Toll-like receptor(TLRs)and tumor necrosis factor(TNF),thrombocyte activation,complement and coagulation cascades pathways,Ditan Decoction potentially intervene process of formation of cerebral edema,inflammatory response,angiogenesis,thrombosis and activation of the complement system.In these ways,Ditan Decoction generates therapeutical effects.Conclusions:This research uncovers the potential molecular targets,biological function modules,signaling pathways of Kyoto Encyclopedia of Genes and Genomics(KEGG)in Ditan Decoction for treating cerebral infarction,providing theoretical evidence and direction for the further research.

关 键 词：涤痰汤 网络药理学 脑梗死 凝血途径 纤溶系统 炎症反应 补体系统 

分 类 号：R285[医药卫生—中药学]