黄芪多糖对2型糖尿病外周血内皮祖细胞归巢干预作用  

Intervention of Astragalus Polysaccharide on the Homing of Endothelial Progenitor Cells from Type 2 Diabetes

作　　者：王燕国 陈文群[2] 徐寒松[3] 王治义 吴青[3] WANG Yanguo;CHEN Wenqun;XU Hansong;WANG Zhiyi;WU Qing(Neihuang County Chinese Medicine Hospital,Henan Neihuang 456300,China;Anshun People's Hospital Guizhou,Anshun 561000,China;The second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine,Guizhou GuiYang 550000,China)

机构地区：[1]河南省安阳市内黄县中医院,河南安阳456300 [2]贵州省安顺市人民医院,贵州安顺561000 [3]贵州省贵阳中医学院第二附属医院,贵州贵阳550000

出　　处：《中医药临床杂志》2020年第6期1088-1094,共7页Clinical Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30960491);贵州省优秀科技教育人才省长基金[黔省专合字(2009)84号]。

摘　　要：目的:探讨黄芪多糖对2型糖尿病(T2DM)患者内皮祖细胞(EPCs)归巢能力的影响方法:培养、鉴定EPCs;建立裸鼠颈动脉损伤模型;PKH26标记的EPCs;分四组:健康人组、黄芪多糖干预组、糖尿病组(即未干预组)、PBS组,前三组经尾静脉移植相应的PKH26已标记的EPCs,PBS组尾静脉注射PBS液;于3和7d后取四组裸鼠损伤动脉,冰冻切片荧光显微镜下观察移植的细胞在内膜处的表达;纵行剖开后荧光显微镜下观察移植的EPCs定向归巢到血管损伤部位的情况并计数分析。结果:同一时间内,2型糖尿病患者EPCs归巢能力较健康人明显降低(P<0.01);2型糖尿病患者EPCs经黄芪多糖干预后,其归巢能力较未干预的糖尿病人增强(P<0.05)。结论:糖尿病患者EPCs在体内归巢的能力下降;糖尿病患者EPCs给予黄芪多糖干预后能增强其定向归巢到血管损伤部位的能力。Objective:To investigate the effect of Astragalus polysaccharides on the homing ability of endothelial progenitor cells(EPCs)in patients with type 2 diabetes mellitus(T2DM)Methods:EPCs were cultured and identified.PKH26-labeled EPCs were established in nude mice.They were divided into four groups:healthy person group,astragalus polysaccharide intervention group,diabetes group(ie,no intervention group),PBS group,and the first three groups.The corresponding PKH26 labeled EPCs were transplanted,and the PBS group was injected with PBS in the tail vein.After 3 and 7 days,the injured arteries of the four groups of nude mice were taken,and the expression of the transplanted cells in the inner membrane was observed under frozen microscope.Post-fluorescence microscopy was used to observe the homing of transplanted EPCs to the site of vascular injury and to count the analysis.Results:At the same time,the homing ability of EPCs in type 2 diabetic patients was significantly lower than that in healthy controls(P<0.01).The homing ability of EPCs in type 2 diabetic patients after intervention with Astragalus polysaccharides was enhanced compared with uninterrupted diabetics(P<0.05).Conclusion:The ability of EPCs to homing in the body of diabetic patients is decreased.The EPCs in diabetic patients can enhance the ability of directional homing to vascular injury sites after intervention with Astragalus polysaccharides.

关 键 词：黄芪多糖(APS) 内皮祖细胞(EPCs) 归巢 

分 类 号：R259[医药卫生—中西医结合]