EGB761对脉冲噪声所致大鼠听力损伤的保护效应及机制研究  

Research on Protective Effects and Mechanisms of EGB761 on Impulse Noise Induced Cochlear Function Damage in Rats

作　　者：韩浩伦 高新军 吴玮 王刚 陈娜 范欣[1,4] 董颖婕 李保卫[2] 刘钢 王磊 孙喆喆[1,2] 屈昌北 Han Haolun;Gao Xinjun;Wu Wei;Wang Gang;Chen Na;Fan Xin;Dong Yingjie;Li Baowei;Liu Gang;Wang Lei;Sun Zhezhe;Qu Changbei(不详;Department of Otolaryngology Head and Neck Surgery,Specialized Medical Center of PLA Strategic Support Force,Beijing 100101,China;Environmental Protection Key Laboratory of Environmental Sense Organ Stress and Health,Beijing 100101,China)

机构地区：[1]战略支援部队特色医学中心耳鼻咽喉科,北京100101 [2]国家环境保护环境感官应激与健康重点实验室,北京100101 [3]战略支援部队特色医学中心医学工程科,北京100101 [4]北京大学战略支援部队特色医学中心教学医院,北京100101 [5]中国航天员科研训练中心,北京100094

出　　处：《航天医学与医学工程》2020年第3期203-207,共5页Space Medicine & Medical Engineering

基　　金：全军十二五重大课题子课题(AWS11J003)。

摘　　要：目的探讨EGB761注射液对脉冲噪声性耳聋大鼠听功能的保护作用及其作用机制。方法36只雄性SD大鼠,空白对照组6只不予任何处理,其余30只同一脉冲噪声暴露后,随机分为3组:单纯噪声组、噪声+生理盐水组和噪声+EGB761组,每组各10只。噪声+EGB761组大鼠给予腹腔注射EGB761(3mL/kg,2/日,连用7日),噪声+生理盐水组注射等量生理盐水。采用听性脑干反应(ABR)检测各组大鼠听力情况,实时定量PCR法检测耳蜗中miR-183表达,HE染色研究耳蜗病理改变。结果单纯噪声组、噪声+生理盐水组、噪声+EGB761组大鼠ABR阈值均显著高于对照组(P<0.05);噪声+EGB761组大鼠ABR阈值明显低于单纯噪声组和噪声+盐水组(P<0.05)。单纯噪声组和噪声+生理盐水组均存在耳蜗渗血改变,而空白组和噪声+EGB761组耳蜗未见明显血性渗出及结构损坏。实时定量PCR结果显示,给予脉冲噪声刺激后,miR-183相对表达量较刺激前下降(P<0.05),EGB761治疗后相对表达量呈上升趋势(P<0.05)。结论EGB761可以减少脉冲噪声对耳蜗的损伤,上调miR-183表达,对耳蜗具有保护作用。Objective To investigate the protective effect of EGB761 on hearing function of rats with impulse noise induced deafness and its possible mechanisms.Methods Thirty-six male SD rats were selected and 6 served as the blank control group without any treatment.The remaining 30 rats were exposed to the same impulse noise and randomly divided into three groups:the noise group,the noise+saline group and the noise+EGB761 group,with 10 rats in each group.The noise+EGB761 group was given intraperitoneal injection of EGB761(3 mL/kg,2/day,for 7 days),and the noise+saline group was given intraperitoneal injection of saline.After the experiment,auditory brainstem response(ABR)was used to detect the hearing status of rats in each group,the expression of miR-183 in the cochlea was detected by quantitative real-time PCR,and the pathological changes of the cochlea were studied by HE staining.Results The ABR thresholds of rats in the noise group,the noise+saline group,the noise+EGB761 group were higher than those in control group(P<0.05);the ABR thresholds of rats in noise+EGB761 group were lower than those in noise group and noise+saline group(P<0.05).HE staining of the cochlea showed no obvious hemorrhagic exudation and structural damage in the control group and noise+EGB761 group,while there was hemorrhagic change in the noise group and noise+saline group.Real-time PCR results showed that the relative expression of miR-183 decreased after pulsed noise stimulation(P<0.01),and increased significantly after treatment with EGB761(P<0.05).Conclusion EGB761 can reduce the damage of cochlea caused by impulse noise,up-regulate the expression of miR-183 and protect the cochlea of rats.

关 键 词：EGB761 脉冲噪声 听性脑干反应 miR-183 

分 类 号：R852.24[医药卫生—航空、航天与航海医学]