纳米材料四氧化三铁和普鲁士蓝调节卵巢癌细胞干性的研究  

The Impact of IONPs and PBNPs Nanoparticles in Regulating Stemness of Ovarian Carcinoma Cells

作　　者：包雪阳 郑丹凤 梅峰 薛蕊 王玲 张宇[2] 窦骏[1] BAO Xue-yang;ZHENG Dan-feng;MEI Feng;XUE Rui;WANG Ling;ZHANG Yu;DOU Juna(Department of Pathogenic Biology and Immunology,School of Medicine,Southeast University,Nanjing 210000,China;School of Biological Sciences&Medical Engineering,Southeast University,Nanjing 210000,China)

机构地区：[1]东南大学医学院病原生物学与免疫学系,南京210000 [2]东南大学生物科学与医学工程学院,南京210000

出　　处：《中国药学杂志》2020年第11期913-918,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金研究项目资助(81572887);国家重点研发计划项目资助(2017YFA0205502)。

摘　　要：目的探究2种治疗肿瘤常用的载药纳米材料,四氧化三铁(iron oxide nanoparticles,IONPs)和普鲁士蓝(prussian blue nanoparticles,PBNPs),对上皮性卵巢癌(epithelia ovarian cancer,EOC)细胞干性的影响,为纳米材料应用于EOC的治疗提供实验依据。方法分别用IONPs和PBNPs处理SKOV3、HO89102种EOC细胞,通过RT-qPCR和Western blot检测耐药基因表达变化;通过细胞增殖和克隆形成实验检测细胞增殖能力变化;运用流式细胞仪检测干性相关表面分子表达量的变化。结果IONPs和PBNPs在2种细胞株上均可以下调耐药基因ABCG和ALDH1A1在mRNA和蛋白水平上的表达;IONPs在2种细胞株上均使CD44+CD117+亚群的比例降低,而PBNPs在2种细胞株上均使CD44+CD117+亚群的比例升高;IONPs和PBNPs均能显著抑制SKOV3细胞株增殖能力,但IONPs对HO8910细胞株无明显作用。结论IONPs可以显著降低EOC SKOV3、HO89102种细胞株的耐药基因和干细胞亚群,而PBNPs虽然降低了这2种细胞的耐药基因,抑制增殖,但是提高了干细胞亚群,促进癌干细胞的生成。OBJECTIVE To study the effects of iron oxide nanoparticles(IONPs)and prussian blue nanoparticles(PBNPs)on the stemness of epithelial ovarian cancer(EOC)cell lines,which may provide experimental basis and reference significance for the application of nanoparticles in the treatment of EOC.METHODS SKOV3 and HO8910 cell lines were treated with IONPs and PBNPs respectively,then the drug-resistant genes in mRNA and protein levels were detected by RT-PCR and Western blot.The change of cell proliferation ability were detected by cell proliferation and clone formation experiments,and the expression of surface stemness-related molecules were detected by flow cytometer.RESULTS The expression of ABCG2 and ALDH1A1 were both down regulated in SKOV3 and HO8910 cell lines treated with IONPs and PBNPs,respectively,while IONPs decreased the CD44+CD117+subpopulation,and PBNPs increased the CD44+CD117+subpopulation.Compared with the control cells,the proliferation of SKOV3 cells treated with IONPs and PBNPs were significantly reduced,however,there were no effects on HO8910 cell's proliferation after the cells treated with PBNPs.CONCLUSION Drug-resistance genes and stem cell subpopulation of EOC SKOV3/HO8910 cell lines treated with IONPs were markedly reduced.However,PBNPs can improve the stem cell subpopulation and promote the cancer stem cell's characteristic of EOC cells but the drug-resistance genes were decreased.

关 键 词：四氧化三铁 普鲁士蓝 上皮性卵巢癌 干性 

分 类 号：R94[医药卫生—药剂学] R737[医药卫生—药学]